Evaluation of non-instrumented nucleic acid amplification by loop-mediated isothermal amplification (NINA-LAMP) for the diagnosis of malaria in Northwest Ethiopia.
Evaluation of non-instrumented nucleic acid amplification by loop-mediated isothermal amplification (NINA-LAMP) for the diagnosis of malaria in Northwest Ethiopia.
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DOI：
10.1186/s12936-015-0559-9
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发表时间：
2015-01-28
期刊：
影响因子：
3
通讯作者：
Pillai DR
中科院分区：
文献类型：
--
作者：
Sema M;Alemu A;Bayih AG;Getie S;Getnet G;Guelig D;Burton R;LaBarre P;Pillai DR
疟疾是包括埃塞俄比亚在内的撒哈拉以南非洲国家的一个主要公共卫生问题。早期准确的诊断以及及时有效的治疗是预防、控制和消除疟疾的各种工具之一。本研究旨在评估非仪器核酸扩增环介导等温扩增 (NINA-LAMP) 与标准厚膜和薄膜显微镜和巢式 PCR 相比的性能，作为埃塞俄比亚西北部疟疾敏感诊断的金标准。 2014 年 3 月至 7 月，在埃塞俄比亚北贡德尔进行了一项横断面研究。从到科拉迪巴健康中心就诊的疟疾疑似患者中采集了 82 份血液样本，并通过显微镜、NINA-LAMP 和巢式 PCR 分析了疟原虫寄生虫。 NINA-LAMP 方法使用 Loopamp™ Malaria Pan/Pf 检测试剂盒进行，用于使用无电加热器检测疟原虫属（更具体地说是恶性疟原虫）的 DNA。将 NINA-LAMP 和显微镜检查的诊断准确性结果指标（分析灵敏度、特异性、预测值和 Kappa 评分）与巢式 PCR 进行比较。初步分析共测试了 82 个样品。使用巢式 PCR 作为参考，主要 NINA-LAMP 检测检测疟原虫属的灵敏度和特异性分别为 96.8%（95% 置信区间 (CI)，83.2% - 99.5%）和 84.3%（95% CI，71.4% - 92.9%）和 100%（95% CI，75.1% - 100%）恶性疟原虫的检测分别为 81.2%（95% CI，69.9% - 89.6%）。显微镜检查显示疟原虫寄生虫检测的敏感性和特异性分别为 93.6%（95% CI，78.5% - 99.0%）和 98.0%（95% CI，89.5% - 99.7%）。事后重复 NINA-LAMP 分析显示诊断准确性有所提高，与巢式 PCR 性能相当，并且在疟原虫属水平和恶性疟原虫寄生虫检测方面优于显微镜检查。与巢式 PCR 相比，NINA-LAMP 在属和种水平上对疟疾诊断和疟原虫寄生虫感染检测具有高度敏感性。 NINA-LAMP 在检测恶性疟以及与非恶性疟物种的区分方面比显微镜更灵敏，并且可能是在低流行地区根除疟疾的关键诊断方式。
Malaria is a major public health problem in sub-Saharan African countries including Ethiopia. Early and accurate diagnosis followed by prompt and effective treatment is among the various tools available for prevention, control and elimination of malaria. This study aimed to evaluate the performance of non-instrumented nucleic acid amplification loop-mediated isothermal amplification (NINA-LAMP) compared to standard thick and thin film microscopy and nested PCR as gold standard for the sensitive diagnosis of malaria in Northwest Ethiopia. A cross-sectional study was conducted in North Gondar, Ethiopia from March to July 2014. Eighty-two blood samples were collected from malaria suspected patients visiting Kola Diba Health Centre and analysed for Plasmodium parasites by microscopy, NINA-LAMP and nested PCR. The NINA-LAMP method was performed using the Loopamp™ Malaria Pan/Pf detection kits for detecting DNA of the genus Plasmodium and more specifically Plasmodium falciparum using an electricity-free heater. Diagnostic accuracy outcome measures (analytical sensitivity, specificity, predictive values, and Kappa scores) of NINA-LAMP and microscopy were compared to nested PCR. A total of 82 samples were tested in the primary analysis. Using nested PCR as reference, the sensitivity and specificity of the primary NINA-LAMP assay were 96.8% (95% confidence interval (CI), 83.2% - 99.5%) and 84.3% (95% CI, 71.4% - 92.9%), respectively for detection of Plasmodium genus, and 100% (95% CI, 75.1% - 100%) and 81.2% (95% CI, 69.9% - 89.6%), respectively for detection of P. falciparum parasite. Microscopy demonstrated sensitivity and specificity of 93.6% (95% CI, 78.5% - 99.0%) and 98.0% (95% CI, 89.5% - 99.7%), respectively for the detection of Plasmodium parasites. Post-hoc repeat NINA-LAMP analysis showed improvement in diagnostic accuracy, which was comparable to nested PCR performance and superior to microscopy for detection at both the Plasmodium genus level and P. falciparum parasites. NINA-LAMP is highly sensitive for the diagnosis of malaria and detection of Plasmodium parasite infection at both the genus and species level when compared to nested PCR. NINA-LAMP is more sensitive than microscopy for the detection of P. falciparum and differentiation from non-falciparum species and may be a critical diagnostic modality in efforts to eradicate malaria from areas of low endemicity.
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影响因子：
3
作者：
Alemu A;Fuehrer HP;Getnet G;Kassu A;Getie S;Noedl H
通讯作者：
Noedl H
在疟疾流行环境中对疟疾寄生虫血症的高度敏感性检测：在乌干达偏远诊所中新的环路介导的等温扩增试剂盒的性能。
DOI：
10.1093/infdis/jit184
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Hopkins H;González IJ;Polley SD;Angutoko P;Ategeka J;Asiimwe C;Agaba B;Kyabayinze DJ;Sutherland CJ;Perkins MD;Bell D
通讯作者：
Bell D
影响因子：
3
作者：
Alemu, Abebe;Fuehrer, Hans-Peter;Noedl, Harald
通讯作者：
Noedl, Harald
影响因子：
3.2
作者：
Alemu, Abebe;Muluye, Dagnachew;Gebeyaw, Melkamu
通讯作者：
Gebeyaw, Melkamu
影响因子：
9.4
作者：
Polley, Spencer D.;Mori, Yasuyoshi;Sutherland, Colin J.
通讯作者：
Sutherland, Colin J.