Decreased physical function and increased pain sensitivity in mice deficient for type IX collagen.
Decreased physical function and increased pain sensitivity in mice deficient for type IX collagen.
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DOI：
10.1002/art.24783
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发表时间：
2009-09
影响因子：
--
通讯作者：
Setton, Lori A.
中科院分区：
文献类型：
--
作者：
Allen, Kyle D.;Griffin, Timothy M.;Rodriguiz, Ramona M.;Wetsel, William C.;Kraus, Virginia B.;Huebner, Janet L.;Boyd, Lawrence M.;Setton, Lori A.
携带Col9a1基因失活(Col9a1-/-)的小鼠会过早地发生骨关节炎和椎间盘退变。在这项研究中，我们研究了Col9a1/-小鼠的功能和症状变化，这些变化可能与这些病理有关。研究了Col9a1-/-和野生型小鼠的反射、功能障碍(横梁行走、爬杆、挂钢丝、握力)、感觉运动技能(旋转杆)、机械敏感度(冯·弗雷头发)和热敏感度(热板/甩尾)。还分析了步态，以确定速度、步频、对称性、站立时间百分比、步长和步幅。尸检后，对血清进行透明质酸分析，并对膝盖和脊柱进行组织学分级，以确定其变性程度。Col9a1-/-小鼠出现代偿性步态改变，机械敏感度增加，身体能力受损。Col9a1-/--小鼠行走的步态特征是站立时间百分比增加，步幅缩短。这些小鼠的机械敏感度也很高，在接触扶正、钢丝悬挂、转杆和爬杆方面存在缺陷。雄性-Col9a1-/-小鼠的平均血清透明质酸水平最高，并且有强有力的软骨侵蚀的组织学证据。还检测到了椎间盘退变，Col9a1/-小鼠的椎间盘撕裂发生率增加。这些数据描述了一种Col9a1/-行为表型，其特征是步态改变，机械敏感性增加，功能受损。这些步态和功能的差异表明，Col9a1/-小鼠选择了限制关节负荷的运动行为。男性的行为变化的性质和幅度最大，也是膝关节退行性变的最大证据。这些发现表明，Col9a1-/-小鼠的行为变化与骨关节炎和椎间盘退变的解剖学迹象一致。
Mice with Col9a1 gene inactivation (Col9a1-/-) prematurely develop osteoarthritis and intervertebral disc degeneration. In this study, we investigate Col9a1-/- mice for functional and symptomatic changes that may associate with these pathologies. Col9a1-/- and wildtype mice were investigated for reflexes, functional impairments, (beam walking, pole climbing, wire-hang, grip-strength), sensorimotor skills (rotarod), mechanical sensitivity (von Frey hair) and thermal sensitivity (hot plate/tail flick). Gait was also analyzed to determine velocity, stride frequency, symmetry, percentage stance times, stride lengths, and step widths. Post-mortem, serum was analyzed for hyaluronan, and knees and spines were graded histologically for degeneration. Col9a1-/- mice had compensatory gait changes, increased mechanical sensitivity, and impaired physical ability. Col9a1-/- mice ambulated with gaits characterized by increased percentage stance times and shorter stride lengths. These mice also had heightened mechanical sensitivity and were deficient in contact righting, wire-hang, rotarod, and pole climbing. Male-Col9a1-/- mice had the highest mean serum hyaluronan levels and strong histological evidence of cartilage erosion. Intervertebral disc degeneration was also detected, with Col9a1-/- mice having increased incidence of disc tears. These data describe a Col9a1-/- behavioral phenotype characterized by altered gait, increased mechanical sensitivity, and impaired function. These gait and functional differences suggest Col9a1-/- mice select locomotive behaviors that limit joint loads. The nature and magnitude of behavioral changes were largest in males, which also had the greatest evidence of knee degeneration. These findings suggest that Col9a1-/- mice present behavioral changes consistent with anatomic signs of osteoarthritis and intervertebral disc degeneration.
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影响因子：
4.9
作者：
Carlsen S;Nandakumar KS;Holmdahl R
通讯作者：
Holmdahl R
影响因子：
2.7
作者：
Higashino, K.;Matsui, Y.;Yasui, N.
通讯作者：
Yasui, N.
IX 型胶原蛋白对正常听力至关重要
影响因子：
3.3
作者：
Asamura, K;Abe, S;Usami, S
通讯作者：
Usami, S
影响因子：
--
作者：
Elliott, AL;Kraus, VB;Jordan, JM
通讯作者：
Jordan, JM
DOI：
10.1097/01.phm.0000056987.33630.56
发表时间：
2003-04-01
影响因子：
3
作者：
Chen, CPC;Chen, MJL;Tang, SFT
通讯作者：
Tang, SFT