Jaceosidin inhibits contact hypersensitivity in mice via down-regulating IFN-γ/STAT1/T-bet signaling in T cells.
Jaceosidin inhibits contact hypersensitivity in mice via down-regulating IFN-γ/STAT1/T-bet signaling in T cells.
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Jaceosidin 通过下调 T 细胞中的 IFN-γ/STAT1/T-bet 信号传导来抑制小鼠的接触性超敏反应。
DOI：
10.1016/j.ejphar.2010.10.068
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发表时间：
2011-01
影响因子：
5
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究旨在研究毛蒿黄酮类化合物jaceosidin在体内外对T淋巴细胞的免疫抑制作用，并进一步探讨其可能的分子机制。Jaceosidin可浓度依赖性地抑制刀豆球蛋白A（ConA）诱导的T细胞增殖和活化，并抑制活化T细胞分泌IL-2、TNF-α和IFN-γ等促炎细胞因子。进一步的研究表明，jaceosidin下调活化的T细胞中STAT 1的活化和T-bet的表达。此外，为了研究jaceosidin在体内的免疫抑制作用，在BALB/c小鼠上进行苦基氯（picryl chloride，PCl）诱导的耳接触性皮炎模型。Jaceosidin以剂量依赖性方式显著改善PCl诱导的耳肿胀，这是由于其抑制STAT 1/T-bet信号通路。总之，这些发现表明，jaceosidin通过抑制T细胞增殖和活化在体外和体内发挥其免疫抑制作用，这与其有效下调IFN-γ/STAT 1/T-bet信号通路密切相关。
In the present study, we aimed to investigate the immunosuppressive activity of jaceosidin, a flavone isolated from Artemisia vestita, on T lymphocytes both in vitro and in vivo, and further explore its potential molecular mechanism. Jaceosidin exerted a significant inhibition on the T cell proliferation and activation induced by concanavalin A (Con A) in a concentration-dependent manner and it also inhibited the secretion of the proinflammatory cytokines such as IL-2, TNF-α and IFN-γ of activated T cells. Further study showed that jaceosidin down-regulated STAT1 activation and T-bet expression in activated T cells. Moreover, in order to investigate the immunosuppressive effect of jaceosidin in vivo, the picryl chloride (PCl)-induced ear contact dermatitis model was performed on BALB/c mice. Jaceosidin significantly ameliorated PCl-induced ear swelling in a dose-dependent manner, which was due to its inhibition of the STAT1/T-bet signaling pathway. In summary, these findings suggest that jaceosidin exerts its immunosuppressive effect both in vitro and in vivo through inhibiting T cell proliferation and activation, which is closely associated with its potent down-regulation of the IFN-γ/STAT1/T-bet signaling pathway.
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
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作者：
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