Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit
Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit
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基于金刚石-金纳米复合材料的多氯联苯电化学适体传感器实现亚飞摩尔检测限
DOI：
10.1021/acsomega.0c02846
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发表时间：
2020-08
期刊：
影响因子：
4.1
通讯作者：
Yibo Ma
中科院分区：
文献类型：
--
作者：
Xiaoxi Yuan;Zhigang Jiang;Qiliang Wang;Nan Gao;Hongdong Li;Yibo Ma
多氯联苯（PCBs）具有高毒性、低致死量和生物富集性，其在广泛领域的应用受到抑制，因此非常需要高效的痕量检测。在这项研究中，我们通过在掺硼金刚石（BDD）上进行两次溅射和两次退火（T-Au-NP）来生产致密的金纳米颗粒。通过扫描电子显微镜、X射线衍射、拉曼光谱和X射线光电子能谱分析证实了T-Au-NPs/BDD纳米复合材料的成功形成。基于T-Au-NPs/BDD，制备了带有适配体的电子生物传感器，通过电化学阻抗检测痕量多氯联苯-77（PCB-77）。基于该生物传感器，在飞摩尔至微摩尔范围内实现了良好的线性关系，并且实现了亚飞摩尔水平的极低检测限（0.32 fM）。这项研究的重点在于金刚石基底在生物传感器中的关键作用。实验结果表明，该生物传感器具有良好的灵敏度、特异性、稳定性和可循环性，有利于检测痕量PCB-77分子。这归因于BDD底物所呈现的重要作用以及T-Au-NPs与适体结合的协同影响。
Polychlorinated biphenyls (PCBs) with high toxicity, low lethal dose, and bioaccumulation have been inhibited for application in wide fields, and a highly efficient trace detection is thus greatly desirable. In this study, we produce dense Au-nanoparticles by twice sputtering and twice annealing (T-Au-NPs) on boron-doped diamond (BDD). The successful formation of T-Au-NPs/BDD nanocomposites was confirmed by scanning electron microscopy, X-ray diffraction, Raman spectroscopy, and X-ray photoelectron spectroscopy analysis. Based on T-Au-NPs/BDD, an electronic biosensor with aptamers is fabricated to detect trace polychlorinated biphenyl-77 (PCB-77) by electrochemical impedance. A good linear relationship in the range of femtomolar to micromolar and significantly low detection limit of sub-femtomolar level (0.32 fM) are realized based on the biosensor. The emphasis of this research lies in the key role of the diamond substrate in the biosensor. It is demonstrated that the biosensor has excellent sensitivity, specificity, stability, and recyclability, which are favorable for detecting the trace PCB-77 molecule. It is attributed to the important effect presented by the BDD substrate and the synergistic influence of T-Au-NPs combined with aptamers.
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影响因子：
5.4
作者：
B. Roustom;G. Foti;C. Comninellis
通讯作者：
B. Roustom;G. Foti;C. Comninellis
用于超灵敏测定持久性有机污染物 (POP) 和剧毒无机离子的多功能环境阻抗传感器
影响因子：
15.1
作者：
Chen, Xing;Guo, Zheng;Liu, Zhong-Gang;Jiang, Yu-Jing;Zhan, Dong-Ping;Liu, Jin-Huai;Huang, Xing-Jiu
通讯作者：
Huang, Xing-Jiu
影响因子：
8.4
作者：
Yihan Wang;Fu-ting Wang;Zi-wei Han;Ke-Jing Huang;Xuemei Wang;Zhen-hua Liu;Shu-yu Wang;Yun-fei Lu
通讯作者：
Yihan Wang;Fu-ting Wang;Zi-wei Han;Ke-Jing Huang;Xuemei Wang;Zhen-hua Liu;Shu-yu Wang;Yun-fei Lu
影响因子：
8.9
作者：
C. Salice;C. L. Rowe;K. Eisenreich
通讯作者：
C. Salice;C. L. Rowe;K. Eisenreich
影响因子：
12.6
作者：
Ziping Zhang;Cancan Tao;Jungang Yin;Yunhui Wang;Yanshen Li
通讯作者：
Ziping Zhang;Cancan Tao;Jungang Yin;Yunhui Wang;Yanshen Li