Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation.
Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation.
复制标题
DOI：
10.3389/fmicb.2020.552135
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Silvano E;Yang M;Wolterink M;Giebel HA;Simon M;Scanlan DJ;Zhao Y;Chen Y
海洋玫瑰杆菌分支附属集群（RCA）代表了全球海洋中最丰富的浮游细菌群之一，特别是在温带和亚极地地区。它们在各种元素的生物地球化学循环中起着关键作用，是海洋气候活性微量气体代谢的重要参与者。RCA细菌是真正的远洋细菌，具有较小的基因组，与富营养化玫瑰杆菌（如pomeroyi Ruegeria DSS-3和Phaeobacter sp. MED193）相比，RCA细菌是真正的富营养化玫瑰杆菌。我们之前的研究表明，RCA细菌似乎不编码plcp介导的脂质重塑途径，即海洋异养细菌通过用替代糖脂或甜菜碱脂替代膜甘油磷脂来重塑膜脂组成，以响应磷(P)胁迫。在这项研究中，我们报告了6个RCA分离株的脂质组学分析。除了常见的甘油磷脂，如磷脂酰甘油（PG）和磷脂酰乙醇胺（PE）， RCA细菌合成一种相对罕见的磷脂，酰基磷脂酰甘油，这是在共养玫瑰杆菌中没有发现的。相反，与丰富的SAR11分支一样，RCA细菌在P胁迫下上调鸟氨酸脂质生物合成，这表明这种氨基酸在海洋异养生物适应海洋营养限制方面发挥了关键作用。
The marine roseobacter-clade affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodeling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), RCA bacteria synthesize a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation.
登录
查看更多内容
DOI：
10.1038/ismej.2015.172
发表时间：
2016-04
期刊：
The ISME journal
影响因子：
--
作者：
Sebastián M;Smith AF;González JM;Fredricks HF;Van Mooy B;Koblížek M;Brandsma J;Koster G;Mestre M;Mostajir B;Pitta P;Postle AD;Sánchez P;Gasol JM;Scanlan DJ;Chen Y
通讯作者：
Chen Y
影响因子：
11
作者：
Voget, Sonja;Wemheuer, Bernd;Simon, Meinhard
通讯作者：
Simon, Meinhard
感染海洋玫瑰杆菌 RCA 谱系的多种、丰富的新型病毒
影响因子：
6.4
作者：
Zhang, Zefeng;Chen, Feng;Zhao, Yanlin
通讯作者：
Zhao, Yanlin
影响因子：
11
作者：
Carini, Paul;Steindler, Laura;Giovannoni, Stephen J.
通讯作者：
Giovannoni, Stephen J.
影响因子：
28.3
作者：
Billerbeck, Sara;Wemheuer, Bernd;Simon, Meinhard
通讯作者：
Simon, Meinhard