Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients.
Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients.
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DOI：
10.1126/sciimmunol.abe5511
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发表时间：
2020-10-08
影响因子：
24.8
通讯作者：
Gingras AC
中科院分区：
文献类型：
--
作者：
Isho B;Abe KT;Zuo M;Jamal AJ;Rathod B;Wang JH;Li Z;Chao G;Rojas OL;Bang YM;Pu A;Christie-Holmes N;Gervais C;Ceccarelli D;Samavarchi-Tehrani P;Guvenc F;Budylowski P;Li A;Paterson A;Yue FY;Marin LM;Caldwell L;Wrana JL;Colwill K;Sicheri F;Mubareka S;Gray-Owen SD;Drews SJ;Siqueira WL;Barrios-Rodiles M;Ostrowski M;Rini JM;Durocher Y;McGeer AJ;Gommerman JL;Gingras AC
唾液是血清的替代生物流体，用于检测和监测COVID-19中SARS-CoV-2刺突和RBD抗原的IgG。虽然对SARS-CoV-2的抗体反应在血液中进行了广泛的研究，但对唾液中的抗体反应及其与全身抗体水平的关系知之甚少。在这里，我们通过酶联免疫吸附测定（ELISA）分析了与阴性对照相比，急性和恢复期实验室诊断的COVID-19患者在症状发作（PSO）后3-115天的血清和唾液中对SARS-CoV-2刺突蛋白（全长三聚体）及其受体结合结构域（RBD）的IgG，伊加和IgM反应。抗SARS-CoV-2抗体应答在血清和唾液中很容易检测到，在PSO 16-30天达到峰值IgG水平。纵向分析显示，抗SARS-CoV-2伊加和IgM抗体迅速衰减，而IgG抗体在两种生物流体中保持相对稳定长达105天PSO。最后，血清中对加标和RBD的IgG、IgM和较小程度的伊加反应与匹配的唾液样本正相关。这项研究证实，血清和唾液中的SARS-CoV-2 IgG抗体在大多数COVID-19患者中至少维持3个月PSO。唾液中的IgG应答可作为SARS-CoV-2全身免疫的替代指标，这是基于其与血清IgG应答的相关性。
Saliva is an alternative biofluid to serum for detecting and monitoring IgG to SARS-CoV-2 spike and RBD antigens in COVID-19. While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about the antibody response in saliva and its relationship to systemic antibody levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA and IgM responses to the SARS-CoV-2 spike protein (full length trimer) and its receptor-binding domain (RBD) in serum and saliva of acute and convalescent patients with laboratory-diagnosed COVID-19 ranging from 3–115 days post-symptom onset (PSO), compared to negative controls. Anti-SARS-CoV-2 antibody responses were readily detected in serum and saliva, with peak IgG levels attained by 16–30 days PSO. Longitudinal analysis revealed that anti-SARS-CoV-2 IgA and IgM antibodies rapidly decayed, while IgG antibodies remained relatively stable up to 105 days PSO in both biofluids. Lastly, IgG, IgM and to a lesser extent IgA responses to spike and RBD in the serum positively correlated with matched saliva samples. This study confirms that serum and saliva IgG antibodies to SARS-CoV-2 are maintained in the majority of COVID-19 patients for at least 3 months PSO. IgG responses in saliva may serve as a surrogate measure of systemic immunity to SARS-CoV-2 based on their correlation with serum IgG responses.
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影响因子：
15.3
作者：
Humphreys, Ian R;de Trez, Carl;Kinkade, April;Benedict, Chris A;Croft, Michael;Ware, Carl F
通讯作者：
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影响因子：
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DOI：
10.3390/ijerph17072225
发表时间：
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影响因子：
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作者：
Khurshid, Zohaib;Asiri, Faris Yahya Ibrahim;Al Wadaani, Hamed
通讯作者：
Al Wadaani, Hamed
DOI：
10.1073/pnas.1218620110
发表时间：
2013-03-26
影响因子：
11.1
作者：
Li, Zhijie;Michael, Iacovos P.;Rini, James M.
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