A fractionation-based protocol to investigate RNA solubility phase transition during Xenopus oocyte maturation.
A fractionation-based protocol to investigate RNA solubility phase transition during Xenopus oocyte maturation.
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DOI：
10.1016/j.xpro.2023.102830
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发表时间：
2024-03-15
期刊：
影响因子：
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通讯作者：
Yang, Jing
中科院分区：
文献类型：
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作者：
Hwang, Hyojeong;Ma, Meng;Yang, Jing
虽然RNA在体外是可溶的，但当它们被结合到细胞内的一些蛋白质复合体中时，它们的溶解度可能会改变。因此，RNA的溶解度相变表明了RNA的功能和活性的变化。在这里，我们提出了一种评估非洲爪哇卵母细胞成熟过程中RNA溶解度相变的方法。我们描述了样品制备、细胞分离、RNA提取、实时荧光聚合酶链式反应和结果分析的步骤。有关该协议的使用和执行的完整细节，请参考Hwang等人。(2023年)。一种用于评估非洲爪哇卵母细胞和卵的RNA分离的基于分馏的方案用于细胞分离、RNA提取、RT-PCR和结果分析的步骤出版者注：实施任何实验方案都需要遵守当地实验室安全和伦理的机构指南。虽然RNA在体外是可溶的，但当它们被结合到细胞内的一些蛋白质复合体中时，它们的溶解度可能会改变。因此，RNA的溶解度相变表明了RNA的功能和活性的变化。在这里，我们提出了一种评估非洲爪哇卵母细胞成熟过程中RNA溶解度相变的方法。我们描述了样品制备、细胞分离、RNA提取、实时荧光聚合酶链式反应和结果分析的步骤。
While RNAs are soluble in vitro, their solubility may be altered when incorporated into some protein complexes inside the cell. The solubility phase transition of RNAs is thus indicative of changes in the function and activity of RNAs. Here, we present a protocol for the assessment of RNA solubility phase transition during Xenopus oocyte maturation. We describe steps for sample preparation, cell fractionation, RNA extraction, real-time PCR, and analysis of the obtained results. For complete details on the use and execution of this protocol, please refer to Hwang et al. (2023). A fractionation-based protocol for assessing the solubility of RNAs Isolation of Xenopus laevis oocytes and eggs Steps for cell fractionation, RNA extraction, RT-PCR, and analysis of results Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. While RNAs are soluble in vitro, their solubility may be altered when incorporated into some protein complexes inside the cell. The solubility phase transition of RNAs is thus indicative of changes in the function and activity of RNAs. Here, we present a protocol for the assessment of RNA solubility phase transition during Xenopus oocyte maturation. We describe steps for sample preparation, cell fractionation, RNA extraction, real-time PCR, and analysis of the obtained results.
影响因子：
11.8
作者：
Hwang，Hyojeong;Chen，Sijie;Yang，Jing
通讯作者：
Yang，Jing
影响因子：
8.8
作者：
通讯作者：
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