Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.
Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.
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DOI：
10.1016/j.celrep.2022.111802
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发表时间：
2022-12-13
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
mRNA 的不对称定位对于细胞极性和细胞命运的决定至关重要。通过进行分馏 RNA-seq，我们在此报告，大量母体 RNA 与非洲爪蟾卵母细胞中的 ER 相关，但在卵母细胞成熟后释放到细胞质中。我们提供的证据表明，大多数 ER 相关 RNA 结合蛋白 (RBP) 在卵母细胞成熟后仍与 ER 相关。然而，所有分析的 ER 相关 RBP 在卵母细胞成熟后都表现出与其一些靶标 RNA 的结合减少。我们的结果进一步表明，在卵母细胞成熟过程中，ER 发生了大规模重塑，导致动物半球形成广泛的管状 ER 网络，这是成熟卵中 mRNA 不对称定位所必需的。因此，我们的研究结果表明，RNA-ER 关联的动态调节和 ER 的重塑对于发育过程中 RNA 的不对称定位非常重要。黄等人。报告指出，爪蟾卵母细胞成熟过程中 ER 的重塑和 RNA-ER 关联的动态调节对于母体 RNA 的不对称定位至关重要，并促进卵母细胞从休眠状态转变为准备受精的减数分裂成熟卵。
Asymmetric localization of mRNAs is crucial for cell polarity and cell fate determination. By performing fractionation RNA-seq, we report here that a large number of maternal RNAs are associated with the ER in Xenopus oocytes but are released into the cytosol after oocyte maturation. We provide evidence that the majority of ER-associated RNA-binding proteins (RBPs) remain associated with the ER after oocyte maturation. However, all ER-associated RBPs analyzed exhibit reduced binding to some of their target RNAs after oocyte maturation. Our results further show that the ER is remodeled massively during oocyte maturation, leading to the formation of a widespread tubular ER network in the animal hemisphere that is required for the asymmetric localization of mRNAs in mature eggs. Thus, our findings demonstrate that dynamic regulation of RNA-ER association and remodeling of the ER are important for the asymmetric localization of RNAs during development. Hwang et al. report that remodeling of the ER and dynamic regulation of the RNA-ER association during Xenopus oocyte maturation is essential for the asymmetric localization of maternal RNAs and facilitates the transition of oocytes from a dormant state into meiotically mature eggs that are ready for fertilization.
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影响因子：
3.3
作者：
Chang, P;Torres, J;King, ML
通讯作者：
King, ML
影响因子：
4.8
作者：
Hoffman, Alyson M.;Chen, Qiang;Nicchitta, Christopher V.
通讯作者：
Nicchitta, Christopher V.
影响因子：
4.6
作者：
Aguero, Tristan;Jin, Zhigang;Yang, Jing
通讯作者：
Yang, Jing
DOI：
10.1016/0005-2787(66)90020-7
发表时间：
1966-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ECKER, RE;SMITH, LD
通讯作者：
SMITH, LD
影响因子：
2.7
作者：
ECKER, RE;SMITH, LD
通讯作者：
SMITH, LD