Autophagy-disrupted LC3 abundance leads to death of supporting cells of human oocytes.
Autophagy-disrupted LC3 abundance leads to death of supporting cells of human oocytes.
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DOI：
10.1016/j.bbrep.2018.08.002
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发表时间：
2018-09
影响因子：
2.7
通讯作者：
Saito H
中科院分区：
文献类型：
--
作者：
Kang W;Ishida E;Yamatoya K;Nakamura A;Miyado M;Miyamoto Y;Iwai M;Tatsumi K;Saito T;Saito K;Kawano N;Hamatani T;Umezawa A;Miyado K;Saito H
细胞部分的自噬再循环通常被称为细胞死亡的反面。在这里，我们通过检查控制卵母细胞质量的颗粒细胞层来探索细胞死亡和自噬之间的关系，这对受精的成功很重要。从不孕妇女收集颗粒细胞层，并在形态学上分为四种类型，即，成熟（MCC）、未成熟（ICC）和发育不良的卵丘细胞（DCC）以及壁颗粒细胞（MGCs）。微管相关蛋白轻链3（LC 3），这是参与自噬体的形成，过度表达在DCC和MGCs，其染色体DNA高度片段化。然而，自噬起始仅限于MGCs，如膜结合的LC 3-II和自噬相关蛋白7（ATG 7）（一种将LC 3-I转化为LC 3-II的酶）的表达所示。虽然pro-LC 3积累，但自噬在DCC中被禁用，导致细胞死亡。我们的研究结果表明，自噬独立的积累的pro-LC 3蛋白导致周围的卵母细胞的人颗粒细胞的死亡，并推测降低卵母细胞的质量和女性生育能力的可能性。LC 3在MGCs和DCC中的表达显著高。典型的自噬仅见于MGCs。DCC中ATG 7表达和溶酶体数量较低。不同的细胞死亡模式发生在MGCs和DCC。
Autophagic recycling of cell parts is generally termed as the opposite of cell death. Here, we explored the relation between cell death and autophagy by examining granulosa cell layers that control oocyte quality, which is important for the success of fertilization. Granulosa cell layers were collected from infertile women and morphologically divided into four types, viz., mature (MCCs), immature (ICCs), and dysmature cumulus cells (DCCs), and mural granulosa cells (MGCs). Microtubule-associated protein light chain 3 (LC3), which is involved in autophagosome formation, was expressed excessively in DCCs and MGCs, and their chromosomal DNA was highly fragmented. However, autophagy initiation was limited to MGCs, as indicated by the expression of membrane-bound LC3-II and autophagy-related protein 7 (ATG7), an enzyme that converts LC3-I to LC3-II. Although pro-LC3 was accumulated, autophagy was disabled in DCCs, resulting in cell death. Our results suggest the possibility that autophagy-independent accumulation of pro-LC3 proteins leads to the death of human granulosa cells surrounding the oocytes and presumably reduces oocyte quality and female fertility. LC3 expression was remarkably high in MGCs and DCCs. Typical autophagy was seen only in MGCs. ATG7 expression and number of lysosomes were low in DCCs. Distinct modes of cell death occurred in MGCs and DCCs.
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凋亡对细胞死亡的形态学和细胞化学测定。
影响因子：
2.3
作者：
Taatjes, Douglas J.;Sobel, Burton E.;Budd, Ralph C.
通讯作者：
Budd, Ralph C.
影响因子：
64.8
作者：
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通讯作者：
Ohsumi, Y
影响因子：
5.8
作者：
Duprez, Linde;Wirawan, Ellen;Vandenabeele, Peter
通讯作者：
Vandenabeele, Peter
DOI：
10.1083/jcb.200412022
发表时间：
2005-05-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Komatsu M;Waguri S;Ueno T;Iwata J;Murata S;Tanida I;Ezaki J;Mizushima N;Ohsumi Y;Uchiyama Y;Kominami E;Tanaka K;Chiba T
通讯作者：
Chiba T
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA