Morphological and cytochemical determination of cell death by apoptosis.

Morphological and cytochemical determination of cell death by apoptosis.
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凋亡对细胞死亡的形态学和细胞化学测定。

DOI:
10.1007/s00418-007-0356-9
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发表时间:
2008-01
影响因子:
2.3
通讯作者:
Budd, Ralph C.
Budd, Ralph C.
中科院分区:
生物学3区
文献类型:
--
作者:
Taatjes, Douglas J.;Sobel, Burton E.;Budd, Ralph C.

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现在已经认识到细胞死亡的几种模式,包括坏死、细胞凋亡和自噬。通常,这些不同模式之间的区别可能并不明显,尽管精确的模式可能在生理上很重要。因此,通常希望能够对细胞死亡的模式进行分类。细胞凋亡最初是通过透射光和电子显微镜观察到的细胞结构改变来定义的。如今,多种成像和细胞化学技术可用于细胞凋亡的研究。本综述将重点介绍其中许多方法,并对它们在培养物和组织中特异性鉴定凋亡细胞方面的优缺点进行评论。
Several modes of cell death are now recognized, including necrosis, apoptosis, and autophagy. Oftentimes the distinctions between these various modes may not be apparent, although the precise mode may be physiologically important. Accordingly, it is often desirable to be able to classify the mode of cell death. Apoptosis was originally defined by structural alterations in cells observable by transmitted light and electron microscopy. Today, a wide variety of imaging and cytochemical techniques are available for the investigation of apoptosis. This review will highlight many of these methods, and provide a critique on the advantages and disadvantages associated with them for the specific identification of apoptotic cells in culture and tissues.
DOI: 10.1016/j.cellbi.2005.08.003
发表时间: 2005-11-01
影响因子: 3.9
作者:
Bilyy, R;Kit, Y;Stoika, R
通讯作者: Stoika, R
DOI: 10.1016/j.neuroscience.2006.10.039
发表时间: 2007-02-09
期刊: NEUROSCIENCE
影响因子: 3.3
作者:
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发表时间: 2005-09-27
期刊: LANGMUIR
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发表时间: 1991-01-01
期刊: CYTOMETRY
影响因子: --
作者:
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DOI: 10.1177/002215540205000401
发表时间: 2002-04-01
影响因子: 3.2
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通讯作者: Willingham, MC