RNA-Sequencing Analysis of Gene-Expression Profiles in the Dorsal Gland of Alligator sinensis at Different Time Points of Embryonic and Neonatal Development.
RNA-Sequencing Analysis of Gene-Expression Profiles in the Dorsal Gland of Alligator sinensis at Different Time Points of Embryonic and Neonatal Development.
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扬子鳄背腺胚胎和新生儿发育不同时间点基因表达谱的RNA测序分析
DOI：
10.3390/life12111787
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发表时间：
2022-11-04
期刊：
影响因子：
3.2
通讯作者：
Wu, Xiaobing
中科院分区：
文献类型：
--
作者：
Nie, Haitao;Zhang, Yuqian;Duan, Shulong;Zhang, Ying;Xu, Yunlu;Zhan, Jixiang;Wen, Yue;Wu, Xiaobing
皮肤衍生物，如表皮腺、角蛋白和源自表皮或真皮的骨结构，通常存在于各种脊椎动物物种中，并且被认为具有与物种的生活方式密切相关的各种功能。然而，大多数爬行动物的腺体皮肤衍生物由于对陆地干燥环境的适应，除了少数具有特定功能的皮肤腺体外，都发生了明显的退化。在鳄鱼的背中线中轴肌肉系统中，有一种称为背腺的椭圆形器官/组织，从颈中部区域延伸到前尾区域，包括扬子鳄，扬子鳄是23种现有鳄鱼中的一种极度濒危物种。在这里，我们提出了第一次调查，专注于特定状态的细胞增殖和细胞凋亡，我们提供了直接的分子证据，支持推测，它可能作为一个全分泌分泌，如在其他已知的多细胞外分泌腺。此外，对扬子鳄胚胎和新生期不同时间点基因表达谱的高通量分析表明，扬子鳄可能通过溶酶体胞吐作用转运和沉积色素和脂质，这些功能可能与孵化前后从羊水环境向陆地环境过渡的适应性功能有关。相应的结果有相当大的重要性，在丰富我们的理解之间的内在关系的皮肤衍生物和生活方式的鳄鱼。为扬子鳄的饲养管理提供了理论依据。背腺（dorsal gland，DG）是位于鳄鱼背中线两侧的一种椭圆形器官/组织，其形态学研究取得了重大进展。本研究利用RNA测序技术（RNA-seq）对扬子鳄胚胎31天（E31）至孵化1天（NH 1）不同时间点的DGs进行了分析。对参与黑素生成、胆固醇代谢和细胞凋亡途径的差异表达基因（DEGs）的转录水平的研究表明，DG可能是一个功能性分泌腺，参与色素的形成、转运和沉积，并通过溶酶体胞吐作用分泌脂质。此外，显着的增殖细胞核抗原（PCNA）和B细胞淋巴瘤2（Bcl-2）阳性信号的免疫组化染色在基底细胞，在平行的免疫反应性TdT介导的dUTP缺口末端标记（TUNEL）在基底上细胞内，提供了直接的分子证据支持的推测，DG作为一种全分泌分泌模式。最后，后续的系统发育和免疫组织化学分析PITX 2，在RNA-seq中确定的DEG，有助于进一步阐明候选基因的转录调控机制。总之，目前的研究结果是相当重要的，在丰富我们的理解之间的内在关系的皮肤衍生物和生活方式的新生扬子鳄sinesis。
Skin derivatives, such as integumentary glands, keratin, and bony structures deriving from the epidermis or dermis, are commonly found in various vertebrates species and are suggested to possess a variety of functions that are closely related to the lifestyle of the species. However, most reptile glandular skin derivatives have undergone obvious degeneration due to environmental adaptation to dry land, with the exception of a few skin glands with specific functions. An oval organ/tissue called the dorsal gland spreading from the mid-cervical region to the anterior caudal region in the axial musculature on the dorsal mid-line has been reported in crocodilians, including Alligator sinensis, which is a critically endangered species of the 23 existing crocodilian species. Here, we present the first investigation to focus on particular states of cell proliferation and cell apoptosis, we provide direct molecular evidence which supports the speculation that it might serve as a holocrine secretory, as observed in the other known multicellular exocrine glands. Furthermore, the high-throughput analysis of gene-expression profiles at different timepoints of embryonic and neonatal Alligator sinensis suggested that it might function through the transport and deposition of pigment and lipids via lysosomal exocytosis, these function might relate to an adaptive function in the transition from an amniotic fluid environment to the terrestrial environment around hatching. The corresponding results have considerable importance in enriching our understanding of the intrinsic relationship between the skin derivatives and lifestyles of crocodilians. Furthermore, they have provided a theoretical basis for the rearing and management of newborn Alligator sinensis. Significant advances have been made in the morphological observations of the dorsal gland (DG), an oval organ/tissue which lies on both sides of the dorsal midline of the crocodilian. In the current study, RNA sequencing (RNA-seq) was used to identify the changing patterns of Alligator sinesis DGs at different timepoints from the 31st embryonic day (E31) to the newly hatched 1st day (NH1). A comprehensive transcriptional changes of differentially expression gene (DEGs) involved in the melanogenesis, cholesterol metabolism, and cell apoptosis pathways suggested that the DG might serves as a functional secretory gland in formation, transport and deposition of pigment, and lipids secretion via lysosomal exocytosis. Furthermore, the remarkable immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and B-cell lymphoma 2 (Bcl-2)-positive signals in the basilar cells, in parallel with the immuno-reactive TdT-mediated dUTP nick-End labeling(TUNEL) within suprabasal cells, provided direct molecular evidence supporting for the speculation that DG serves as a holocrine secretion mode. Finally, subsequent phylogenetic and immunohistochemical analysis for the PITX2, the identified DEGs in the RNA-seq, was helpful to further elucidate the transcriptional regulatory mechanism of candidate genes. In conclusion, the current results are of considerable importance in enriching our understanding of the intrinsic relationship between the skin derivatives and lifestyles of newborn Alligator sinesis.
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影响因子：
2.7
作者：
Jin Y;Weinstein DC
通讯作者：
Weinstein DC
DOI：
10.1002/ar.22645
发表时间：
2013-03-01
影响因子：
2
作者：
Liman, Narin;Alan, Emel
通讯作者：
Alan, Emel
影响因子：
3.7
作者：
BRAVO, R
通讯作者：
BRAVO, R
DOI：
10.1152/ajpendo.00086.2003
发表时间：
2003-07-01
影响因子：
5.1
作者：
Juhl, K;Hoy, M;Gromada, J
通讯作者：
Gromada, J
影响因子：
3
作者：
Heldring, Nina;Joseph, Bertrand;Kioussi, Chrissa
通讯作者：
Kioussi, Chrissa