Advanced glycation of type I collagen and fibronectin modifies periodontal cell behavior.
Advanced glycation of type I collagen and fibronectin modifies periodontal cell behavior.
复制标题
DOI：
10.1902/jop.2008.080210
复制
发表时间：
2008-11
影响因子：
4.3
通讯作者：
Steffensen B
中科院分区：
文献类型：
--
作者：
Murillo J;Wang Y;Xu X;Klebe RJ;Chen Z;Zardeneta G;Pal S;Mikhailova M;Steffensen B
晚期糖基化终产物(AGEs)与糖尿病的发病机制有关。然而，蛋白糖基化在糖尿病患者牙周疾病中的作用知之甚少。因此，本研究探讨了I型胶原(Coli)和纤维连接蛋白(FN)的糖基化是否改变了人牙龈成纤维细胞(HGF)和牙周膜成纤维细胞(HPDL)的行为。使用甲基乙二醛(MG)对大肠杆菌和FN进行体外快速糖基化。通过SDS-PAGE和蛋白印迹分析AGEs的形成，并用MG糖化蛋白的特异性抗体进行分析。然后，实验表征了糖化FN和大肠杆菌对HGF和hPDL行为的影响。镁糖化大肠杆菌和FN在不到6小时内。MG诱导的AGEs的特异性抗体与糖化的FN和大肠杆菌反应，但不与对照蛋白反应，这证实了反应的特异性。在细胞培养实验中，糖化的FN支持HGF和hPDL的附着效率显著降低(P<；0.05)。此外，细胞的形态参数，包括长度、面积、周长和形状因子，在两种糖化蛋白上都发生了改变(P<；0.001)。最后，糖化的FN和大肠杆菌上的细胞迁移均减少(P<；0.001)。镁处理能有效地糖化大肠杆菌和FN，为研究糖尿病对牙周病的影响提供了新的工具。糖化大肠杆菌和FN对HGF和hPDL行为的实质性影响表明，蛋白质糖基化参与了糖尿病患者牙周伤口愈合的发病机制和改变。
Advanced glycation end products (AGEs) have been linked to pathogenic mechanisms of diabetes mellitus. However, little is known about the contribution of protein glycation to periodontal disease in patients with diabetes. Therefore, this study investigated whether glycation of type I collagen (COLI) and fibronectin (FN) modified the behavior of human gingival fibroblasts (hGF) and periodontal ligament fibroblasts (hPDL). Procedures for rapid in vitro glycation of COLI and FN used methylglyoxal (MG). Formation of AGEs was analyzed by changes in protein migration using SDS-PAGE and Western blotting with antibodies specific for MG-glycated proteins. Experiments then characterized the effects of glycated FN and COLI on the behavior of hGF and hPDL. MG glycated COLI and FN in less than 6 hours. Confirming the specificity of the reactions, antibodies specific for MG-induced AGEs reacted with glycated FN and COLI, but not with control proteins. In cell culture experiments, glycated FN was significantly less efficient in supporting the attachment of hGF and hPDL (P<0.05). Moreover, the morphological parameters for cells, including length, area, perimeter, and shape factor, were altered (P<0.001) for cells on both glycated proteins. Finally, cell migration was reduced on both glycated FN and COLI (P<0.001). MG treatment efficiently glycated COLI and FN, providing a new tool to study effects of diabetes on periodontal disease. The substantial effects of glycated COLI and FN on hGF and hPDL behavior indicate that protein glycation contributes to the pathogenesis and altered periodontal wound healing observed in patients with diabetes.
登录
查看更多内容
影响因子：
6.4
作者：
ENGVALL, E;RUOSLAHTI, E
通讯作者：
RUOSLAHTI, E
影响因子：
13.5
作者：
Fehrenbach, H;Weiskirchen, R;Gressner, AM
通讯作者：
Gressner, AM
DOI：
10.1073/pnas.93.6.2353
发表时间：
1996-03-19
影响因子：
11.1
作者：
Miyata, T;Taneda, S;Monnier, VM
通讯作者：
Monnier, VM
影响因子：
3.4
作者：
HAIK, GM;LO, TWC;THORNALLEY, PJ
通讯作者：
THORNALLEY, PJ
影响因子：
6
作者：
MCLELLAN, AC;THORNALLEY, PJ;SONKSEN, PH
通讯作者：
SONKSEN, PH