A simple method for gene expression and chromatin profiling of individual cell types within a tissue.
A simple method for gene expression and chromatin profiling of individual cell types within a tissue.
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DOI：
10.1016/j.devcel.2010.05.013
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发表时间：
2010-06-15
影响因子：
11.8
通讯作者：
Henikoff, Steven
中科院分区：
文献类型：
--
作者：
Deal, Roger B.;Henikoff, Steven
了解发育过程中特殊细胞的产生和功能需要分离单个细胞类型进行分析，但这是目前的主要技术挑战。在这里，我们描述了一种特定细胞类型的RNA和染色质图谱的方法，该方法绕过了当前细胞分离方法的许多限制。我们使用体内生物素标记单个细胞类型的核膜蛋白，然后亲和分离标记的核，以测量拟南芥根表皮毛细胞和非毛细胞类型的基因表达和染色质特征。我们确定了数百个在每种细胞类型中优先表达的基因，并表明在毛细胞和非毛细胞之间表达差异最大的基因在组蛋白H3赖氨酸4和27的三甲基化方面也显示出细胞类型的差异。这种方法应该适用于任何能够转化的有机体。
Understanding the production and function of specialized cells during development requires the isolation of individual cell types for analysis, but this is currently a major technical challenge. Here we describe a method for cell type-specific RNA and chromatin profiling that circumvents many of the limitations of current methods for cell isolation. We used in vivo biotin labeling of a nuclear envelope protein in individual cell types followed by affinity isolation of labeled nuclei to measure gene expression and chromatin features of the hair and non-hair cell types of the Arabidopsis root epidermis. We identified hundreds of genes that are preferentially expressed in each cell type and show that genes with the largest expression differences between hair and non-hair cells also show differences between cell types in the trimethylation of histone H3 at lysines 4 and 27. This method should be applicable to any organism that is amenable to transformation.
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影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
5.8
作者：
Baldi, P;Long, AD
通讯作者：
Long, AD
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作者：
Birnbaum, K;Jung, JW;Benfey, PN
通讯作者：
Benfey, PN
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Di Cristina, M;Sessa, G;Morelli, G
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Morelli, G
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作者：
Birnbaum, K;Shasha, DE;Benfey, PN
通讯作者：
Benfey, PN