A Patched-Like Protein PsPTL Is Not Essential for the Growth and Response to Various Stresses in Phytophthora sojae.
A Patched-Like Protein PsPTL Is Not Essential for the Growth and Response to Various Stresses in Phytophthora sojae.
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DOI：
10.3389/fmicb.2021.673784
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发表时间：
2021
影响因子：
5.2
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Xue Z;Wang W;Shen J;Zhang J;Zhang X;Liu X
Patched（Ptc）和Patched related（Ptr）蛋白在真核生物中具有高度保守性，在脂质转运和代谢中起重要作用。通过对大豆疫霉基因组数据库的检索，发现了4个同时含有SSD和Patched结构域的蛋白，其中1个蛋白被鉴定为Patched-like（PTL）蛋白。在此，我们研究了PsPTL的生物学功能。PsPTL在菌丝和孢子形成阶段的表达量高于游动孢子、孢囊和萌发孢囊阶段，但在侵染过程中表达量无明显变化。然而，使用CRISPR/Cas9缺失PsPTL对大豆疫霉的生长、发育或毒力没有显著影响。进一步的研究表明，PsPTL不是大豆疫霉科普外部胁迫如温度、pH、氧化和渗透压所必需的。此外，该基因似乎没有发挥重要作用，大豆疫霉的反应外源甾醇。含有预测的SSD和Patched结构域的其他三种蛋白质的转录水平在PsPTL缺失转化体中也没有显著上调。我们的研究表明，PsPTL不是大豆疫霉的必需蛋白，PsPTL在特定条件下或在其他信号通路中的潜在功能有待进一步研究。
Patched (Ptc) and Patched-related (Ptr) proteins containing sterol-sensing domains (SSD) and Patched domains are highly conserved in eukaryotes for lipid transport and metabolism. Four proteins containing predicted SSD and Patched domains were simultaneously found by searching the Phytophthora sojae genome database, and one of them was identified as a Patched-like (PTL) protein. Here, we investigated the biological function of PsPTL. The expression level of PsPTL was higher during mycelial and sporulation stages, compared to zoospore (ZO), cyst, and germinated-cyst stages, without significant change during infection. However, deletion of PsPTL using CRISPR/Cas9 had no significant effect on the growth, development, or virulence of P. sojae. Further investigations showed that PsPTL is not essential for P. sojae to cope with external stresses such as temperature, pH, oxidative and osmotic pressure. In addition, this gene did not appear to play an essential role in P. sojae’s response to exogenous sterols. The transcript levels of the other three proteins containing predicted SSD and Patched domains were also not significantly upregulated in PsPTL deletion transformants. Our studies demonstrated that PsPTL is not an essential protein for P. sojae under the tested conditions, and more in-depth research is required for revealing the potential functions of PsPTL under special conditions or in other signaling pathways.
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