Impact of 25 years of inorganic fertilization on diazotrophic abundance and community structure in an acidic soil in southern China
Impact of 25 years of inorganic fertilization on diazotrophic abundance and community structure in an acidic soil in southern China
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25年无机施肥对中国南方酸性土壤固氮丰度和群落结构的影响
DOI：
10.1016/j.soilbio.2017.06.019
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发表时间：
2017-10
影响因子：
9.7
通讯作者：
Shen Renfang
中科院分区：
文献类型：
--
作者：
Wang Chao;Zheng Manman;Song Wenfeng;Wen Shilin;Wang Boren;Zhu Chunquan;Shen Renfang
虽然重氮菌在氮素循环中很重要，并对植物有效氮库有贡献，但种群对长期无机肥的反应在很大程度上是未知的。在这里，我们调查了生长在酸性农田土壤中的玉米在体土和根际土壤中的重氮营养种群，这些土壤经历了25年的无机肥。不施肥对照、单施氮肥、氮肥配生石灰、磷(P)、钾(K)肥、N+P+K肥、N+P+K肥+生石灰。采用定量聚合酶链式反应和高通量焦解测序的方法分析了重氮菌的丰度和群落组成。所有肥料处理都提高了土壤养分的有效性，但不施生石灰的处理会导致土壤酸化。玉米生物量和nifH拷贝数在N和N+P+K处理下显著降低，而在P+K处理下显著增加。施生石灰有效缓解了氮素输入的抑制效应。施肥导致了可操作分类单元丰富度的降低和固氮群落组成的转变。土壤pH和养分有效性对固氮丰度有协同作用，而土壤养分有效性似乎是形成固氮群落结构的主要因素。根际效应增加了iIFH基因的拷贝数，但对当前研究尺度上的固氮群落组成没有明显的影响。总体而言，长期无机肥对固氮丰度和群落组成均有影响，且化肥处理对群落组成的影响大于生石灰修复或作物栽培。
Although diazotrophs are important in the nitrogen (N)-cycle and contribute to the pool of plant available N, the population response to long-term inorganic fertilization is largely unknown. Here, we investigated the diazotrophic populations in both the bulk and rhizosphere soils of maize grown in an acidic farmland soil that experienced 25 years of inorganic fertilization. The fertilization regimes included unfertilized control, N fertilizer alone, N fertilizer with quicklime, phosphorus (P) and potassium (K) fertilizers, N + P + K fertilizers, and N + P + K fertilizers with quicklime. Quantitative PCR and high-throughput pyrosequencing of thenifHgene were used to analyze diazotrophic abundance and community composition. All of the fertilizer treatments improved soil nutrient availability, but those without quicklime caused soil acidification. Maize biomasses andnifHcopy numbers were significantly lower under N and N + P + K treatments but increased under P + K fertilization. Quicklime applications effectively alleviated the inhibitory effect of N input. Fertilization led to decreases in operational taxonomic unit richness and shifts in diazotrophic community composition. Soil pH and nutrient availability had a cooperative effect on diazotrophic abundance, while soil nutrient availability appeared to be the main factor shaping diazotrophic community structure. Rhizosphere effects increased thenifHgene copy number but did not obviously change the diazotrophic community composition on the current research scale. Overall, the long-term inorganic fertilization affected both diazotrophic abundance and community composition, and the fertilizer treatment had a greater influence than quicklime remediation or crop cultivation on community composition.
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影响因子：
4.8
作者：
Zhao, Jun;Ni, Tian;Shen, Qirong
通讯作者：
Shen, Qirong
DOI：
10.1093/database/bau001
发表时间：
2014
期刊：
Database : the journal of biological databases and curation
影响因子：
--
作者：
Gaby JC;Buckley DH
通讯作者：
Buckley DH
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Anna K. Simonsen;Shery Han;Philip Rekret;C. Rentschler;Katy D. Heath;J. Stinchcombe
通讯作者：
Anna K. Simonsen;Shery Han;Philip Rekret;C. Rentschler;Katy D. Heath;J. Stinchcombe
影响因子：
5.1
作者：
Weibing Xun;Jun Zhao;Chao Xue;Guishan Zhang;W. Ran;Bo-ren Wang;Q. Shen;Ruifu Zhang
通讯作者：
Weibing Xun;Jun Zhao;Chao Xue;Guishan Zhang;W. Ran;Bo-ren Wang;Q. Shen;Ruifu Zhang
影响因子：
4.9
作者：
S. S. Videira-S.;M. D. C. Pereira E Silva;Péricles Souza Galisa;A. C. F. Dias;R. Nissinen;Vera Lúcia Baldani Divan-Vera-Lúcia-B
通讯作者：
S. S. Videira-S.;M. D. C. Pereira E Silva;Péricles Souza Galisa;A. C. F. Dias;R. Nissinen;Vera Lúcia Baldani Divan-Vera-Lúcia-B