Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells.
Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells.
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成纤维细胞生长因子9对骨髓基质干细胞和牙髓干细胞成骨分化的影响
DOI：
10.3892/mmr.2014.2998
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发表时间：
2015-03
影响因子：
3.4
通讯作者：
Shen SG
中科院分区：
文献类型：
--
作者：
Lu J;Dai J;Wang X;Zhang M;Zhang P;Sun H;Zhang X;Yu H;Zhang W;Zhang L;Jiang X;Shen SG
成纤维细胞生长因子9（FGF 9）在骨形成中的作用可能取决于基因剂量，发育阶段，细胞类型或与其他细胞因子的相互作用。在本研究中，骨髓基质干细胞（BMSCs）和牙髓干细胞（DPSCs）的培养和成骨诱导，在不同浓度的外源性FGF 9处理。采用碱性磷酸酶染色、茜素红S染色、逆转录定量聚合酶链反应和western blot分析成骨标志物的基因表达水平。本研究的结果表明，FGF 9增强细胞外信号调节激酶1/2（ERK 1/2）的磷酸化在成骨诱导过程中的BMSC和DPSC，这是来自不同的组织。FGF 9还通过激活ERK 1/2抑制BMSC和DPSC的成骨分化。这些结果表明，FGF 9可能是体外间充质干细胞成骨的抑制剂，其在体内的应用需要进一步研究。
The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, alizarin red S staining, reverse transcription quantitative polymerase chain reaction and western blot analyses were performed in order to investigate the gene expression levels of osteogenic markers. The results of the present study demonstrated that FGF9 enhanced the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) during osteogenic induction in BMSCs and DPSCs, which are derived from different tissues. FGF9 also inhibited the osteogenic differentiation of BMSCs and DPSCs through the activation of ERK1/2. These findings suggested that FGF9 may be an inhibitor of osteogenesis in mesenchymal stem cells in vitro and its application in vivo requires investigation in the future.
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影响因子：
3.7
作者：
Komada Y;Yamane T;Kadota D;Isono K;Takakura N;Hayashi S;Yamazaki H
通讯作者：
Yamazaki H
影响因子：
14
作者：
Dai, Jiewen;Wang, Jia;Shen, Guofang
通讯作者：
Shen, Guofang
DOI：
10.1073/pnas.0903302106
发表时间：
2009-09-08
影响因子：
11.1
作者：
Nakamura, Tomoki;Gulick, James;Robbins, Jeffrey
通讯作者：
Robbins, Jeffrey
影响因子：
1.2
作者：
Sylvestersen, Kathrine B.;Herrera, Pedro L.;Rescan, Claude
通讯作者：
Rescan, Claude
影响因子：
4.6
作者：
White, Andrew C.;Lavine, Kory J.;Ornitz, David M.
通讯作者：
Ornitz, David M.