Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells.
Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells.
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DOI：
10.1371/journal.pone.0046436
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yamazaki H
中科院分区：
文献类型：
--
作者：
Komada Y;Yamane T;Kadota D;Isono K;Takakura N;Hayashi S;Yamazaki H
间充质细胞起源于神经嵴 (NC) 或中胚层。然而，很难区分NC来源的细胞和中胚层来源的细胞。使用编码 P0-Cre、Wnt1-Cre、Mesp1-Cre 和 Rosa26EYFP 的双转基因小鼠系统，使我们能够追踪 NC 衍生或中胚层衍生细胞作为 YFP 表达细胞，我们首次证明 NC 衍生（P0 或 Wnt1 标记）和中胚层衍生（Mesp1 标记）细胞都有助于发育 从胎儿阶段到成人阶段的牙齿、胸腺和骨髓 (BM) 间充质。无论所涉及的组织如何，NC 衍生的细胞和中胚层衍生的细胞分别主要形成血管周围细胞和内皮细胞。牙齿和胸腺间充质由 NC 衍生的或中胚层衍生的细胞组成，而 BM 间充质的一半由非 NC 或中胚层衍生的细胞组成。然而，集落形成单位成纤维细胞 (CFU-F) 测定表明，牙髓、胸腺和 BM 中的 CFU-F 由 NC 衍生细胞和中胚层衍生细胞组成。二次CFU-F测定用于估计自我更新潜力，结果表明牙齿、胸腺和BM中的CFU-F分别是完全NC衍生的细胞、完全中胚层衍生的细胞和大部分NC衍生的细胞。无论组织及其来源如何，添加抗血小板衍生生长因子受体-β 抗体都会显着抑制集落形成。此外，牙齿间充质表达编码关键造血因子的基因，例如白细胞介素7、干细胞因子和半胱氨酸-X-半胱氨酸（CXC）趋化因子配体12，支持B淋巴细胞和破骨细胞的分化。因此，在这些组织中发现的间充质干细胞具有不同的起源，但在每个器官中具有相似的特性。
Mesenchymal cells arise from the neural crest (NC) or mesoderm. However, it is difficult to distinguish NC-derived cells from mesoderm-derived cells. Using double-transgenic mouse systems encoding P0-Cre, Wnt1-Cre, Mesp1-Cre, and Rosa26EYFP, which enabled us to trace NC-derived or mesoderm-derived cells as YFP-expressing cells, we demonstrated for the first time that both NC-derived (P0- or Wnt1-labeled) and mesoderm-derived (Mesp1-labeled) cells contribute to the development of dental, thymic, and bone marrow (BM) mesenchyme from the fetal stage to the adult stage. Irrespective of the tissues involved, NC-derived and mesoderm-derived cells contributed mainly to perivascular cells and endothelial cells, respectively. Dental and thymic mesenchyme were composed of either NC-derived or mesoderm-derived cells, whereas half of the BM mesenchyme was composed of cells that were not derived from the NC or mesoderm. However, a colony-forming unit-fibroblast (CFU-F) assay indicated that CFU-Fs in the dental pulp, thymus, and BM were composed of NC-derived and mesoderm-derived cells. Secondary CFU-F assays were used to estimate the self-renewal potential, which showed that CFU-Fs in the teeth, thymus, and BM were entirely NC-derived cells, entirely mesoderm-derived cells, and mostly NC-derived cells, respectively. Colony formation was inhibited drastically by the addition of anti-platelet–derived growth factor receptor-β antibody, regardless of the tissue and its origin. Furthermore, dental mesenchyme expressed genes encoding critical hematopoietic factors, such as interleukin-7, stem cell factor, and cysteine-X-cysteine (CXC) chemokine ligand 12, which supports the differentiation of B lymphocytes and osteoclasts. Therefore, the mesenchymal stem cells found in these tissues had different origins, but similar properties in each organ.
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DOI：
10.1016/0922-3371(90)90079-c
发表时间：
1990-05-01
期刊：
CELL DIFFERENTIATION AND DEVELOPMENT
影响因子：
--
作者：
ATSUMI, T;MIWA, Y;IKAWA, Y
通讯作者：
IKAWA, Y
DOI：
10.1111/j.1365-2184.1970.tb00347.x
发表时间：
1970-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
通讯作者：
LALYKINA, KS
影响因子：
56.9
作者：
BOCKMAN, DE;KIRBY, ML
通讯作者：
KIRBY, ML
影响因子：
20.3
作者：
GRONTHOS, S;SIMMONS, PJ
通讯作者：
SIMMONS, PJ
影响因子：
2.7
作者：
McBratney-Owen, B.;Iseki, S.;Morriss-Kay, G. M.
通讯作者：
Morriss-Kay, G. M.