PD-1 Coexpression Gene Analysis and the Regulatory Network in Endometrial Cancer Based on Bioinformatics Analysis.
PD-1 Coexpression Gene Analysis and the Regulatory Network in Endometrial Cancer Based on Bioinformatics Analysis.
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基于生物信息学分析的子宫内膜癌PD-1共表达基因分析及调控网络
DOI：
10.1155/2021/9923434
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发表时间：
2021
影响因子：
--
通讯作者：
Qu P
中科院分区：
文献类型：
--
作者：
Wang L;Liu Z;Zhang W;Zhang A;Qu P
妇科恶性肿瘤是女性生殖系统的肿瘤，主要是宫颈癌、子宫内膜癌和卵巢癌。子宫内膜癌是发达国家最常见的妇科恶性肿瘤。本研究旨在通过生物信息学分析构建程序性细胞死亡蛋白1（programmed cell death protein 1，PD-1）共表达基因网络，筛选PD-1在子宫内膜癌中的潜在生物标志物。此外，还鉴定了参与PD-1和调节肿瘤免疫状态的基因和途径。我们选择TCGA中的EC转录组数据集在cBioPortal平台上检索基因集，并在该平台上获得PD-1共表达基因。使用大卫数据库对共表达基因进行GO和KEGG富集分析。利用Cytoscape 3.7.1软件构建靶蛋白相互作用（PPI）网络，筛选中心基因。共获得976个共表达基因。富集分析显示，PD-1共表达基因在细胞结构的整体组分、细胞因子与细胞因子受体的相互作用、趋化因子信号通路和细胞粘附分子（CAM）中显著富集。通过节点度分析得到10个枢纽基因。CD 3E基因参与EC的预后和免疫过程，其表达水平与PD-1相关（Pearson相关系数为0.82，P < 0.01）。EC中CD 3E基因表达低的患者预后差。PD-1的共表达枢纽基因与免疫相关，其中CD 3E是参与PD-1/PD-L1诱导的肿瘤免疫逃逸的预后标志物。本研究为PD-1/PD-L1在EC中的作用机制及靶向药物的精确治疗提供了新的研究领域。
Gynecological malignancies are tumors of the female reproductive system, mainly cervical cancer, endometrial cancer, and ovarian cancer. Endometrial cancer (EC) is the most common gynecological malignant tumor in developed countries. The aim of this study was to construct a network of programmed cell death protein 1 (PD-1) coexpressed genes through bioinformatics analysis and screen the potential biomarkers of PD-1 in endometrial cancer. In addition, genes and pathways involved in PD-1 and modulating tumor immune status were identified. We select the EC transcriptomic dataset in TCGA to retrieve gene sets on the cBioPortal platform, and the PD-1 coexpressed genes were obtained on the platform. GO and KEGG enrichment analysis of coexpressed genes was performed using the DAVID database. The target protein-protein interaction (PPI) network was constructed using Cytoscape 3.7.1 software, and the hub genes were then screened. A total of 976 coexpression genes were obtained. The enrichment analysis showed that PD-1 coexpressed genes were significantly enriched in overall components of the cell structure, the interaction of cytokines with cytokine receptors, chemokine signaling pathways, and cell adhesion molecules (CAMs). Ten hub genes were obtained by node degree analysis. CD3E gene is involved in the prognosis and immune process of EC, and the expression level is related to PD-1 (Pearson correlation coefficient is 0.82, P < 0.01). Patients with low CD3E gene expression in EC have a poor prognosis. The coexpression hub genes of PD-1 are related to immunity, in which CD3E is a prognostic marker that is involved in the PD-1/PD-L1-induced tumor immune escape. This study provides a new area to study the mechanism of PD-1/PD-L1 in EC and the precise treatment with targeted drugs.
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PD-1和PD-L1检查点信号传导抑制癌症免疫疗法：机制，组合和临床结果。
影响因子：
5.6
作者：
Alsaab HO;Sau S;Alzhrani R;Tatiparti K;Bhise K;Kashaw SK;Iyer AK
通讯作者：
Iyer AK
DOI：
10.1093/bioinformatics/bts251
发表时间：
2012-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Jiao X;Sherman BT;Huang da W;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA
DOI：
10.1126/science.aan5951
发表时间：
2018-02-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Miao D;Margolis CA;Gao W;Voss MH;Li W;Martini DJ;Norton C;Bossé D;Wankowicz SM;Cullen D;Horak C;Wind-Rotolo M;Tracy A;Giannakis M;Hodi FS;Drake CG;Ball MW;Allaf ME;Snyder A;Hellmann MD;Ho T;Motzer RJ;Signoretti S;Kaelin WG Jr;Choueiri TK;Van Allen EM
通讯作者：
Van Allen EM
影响因子：
10.1
作者：
Jie HB;Srivastava RM;Argiris A;Bauman JE;Kane LP;Ferris RL
通讯作者：
Ferris RL
DOI：
10.2147/ccid.s120877
发表时间：
2017
期刊：
Clinical, cosmetic and investigational dermatology
影响因子：
--
作者：
Karlsson AK;Saleh SN
通讯作者：
Saleh SN