Rapid sprouting of filopodia in nerve terminals of chromaffin cells, PC12 cells, and dorsal root neurons induced by electrical stimulation
Rapid sprouting of filopodia in nerve terminals of chromaffin cells, PC12 cells, and dorsal root neurons induced by electrical stimulation
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电刺激诱导嗜铬细胞、PC12细胞和背根神经元神经末梢丝状伪足快速萌发
DOI：
10.1523/jneurosci.14-10-05917.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
S. Terakawa
中科院分区：
文献类型：
--
作者：
S. Manivannan;S. Terakawa
用高倍率(12,000倍)的视频增强差示干涉显微镜研究了直接电刺激神经末梢引起的快速形态变化。我们主要使用培养的牛嗜铬细胞和PC12细胞，牛嗜铬细胞发育成轴突样突起，PC12细胞在NGF处理后显示出神经元分化。在少数病例中，还观察了大鼠背根神经节的初级神经元。短暂的脉冲刺激末端和静脉曲张可引起胞吐，并伴有丝状足的迅速形成。这些丝状伪足直径0.1-0.2微米，长达10微米，在几百毫秒内形成，然后在数十秒内回缩。K去极化也能诱导它们的产生。这种快速的丝状萌发强烈地依赖于细胞外钙离子的存在，并可以在含有钙络合剂(EGTA)或La2+的介质中被取消。抗细胞骨架药物秋水仙碱和细胞松弛素B未能完全阻断这种反应，但利多卡因完全抑制了这种反应。胞吐作用和丝芽萌发的定量分析表明，它们是独立的事件，没有直接联系，它们有不同的阈值，通常丝芽形成的阈值更高。在PC12细胞中，丝状萌发的程度随着细胞分化状态的不同而不同，这表明在其分化的特定阶段快速萌发具有功能作用。重复刺激可以更容易地诱导出丝状伪足。同样的反应可能发生在接近靶细胞的生长锥体，甚至是成熟的突触，特别是在重复电活动之后，可能在使用依赖型突触的形成或可塑性中发挥作用。
Rapid morphological changes induced by direct electrical stimulation of nerve terminals were studied by using video-enhanced differential interference contrast microscopy at a very high magnification (12,000x). We used mainly cultured bovine chromaffin cells, which developed neurite-like processes, and PC12 cells, which showed neuronal differentiation upon NGF treatment. In a few cases, primary neurons of the rat dorsal root ganglion were also examined. Brief pulse stimulation of the terminals and varicosities induced exocytosis accompanied by rapid formation of filopodia. These filopodia, 0.1–0.2 micron in diameter and up to 10 microns in length, formed within a few hundreds of milliseconds and then retracted within tens of seconds. They could also be induced by K depolarization. This rapid filopodial sprouting strongly depended on the presence of extracellular Ca2+ and could be abolished in a medium containing a Ca chelator (EGTA) or La2+. Anti-cytoskeletal agents colchicine and cytochalasin B failed to block this response completely but lidocaine fully suppressed it. Quantitative analysis of exocytosis and filopodial sprouting showed that they were independent events, not directly linked to each other, having different thresholds usually higher for filopodial formation. In PC12 cells, the extent of filopodial sprouting varied with the state of differentiation of the cells, suggesting a functional role of rapid sprouting during a particular phase of their differentiation. Filopodia could be induced with greater ease by repetitive stimulation. The same responses may occur at growth cones approaching the target cells or even at mature synapses particularly after repetitive electrical activity, possibly playing a role in use-dependent synapse formation or plasticity.
影响因子：
56.9
作者：
COHAN, CS;KATER, SB
通讯作者：
KATER, SB
影响因子：
56.9
作者：
BORGENS, RB;ROEDERER, E;COHEN, MJ
通讯作者：
COHEN, MJ
神经元及其效应器官的大分子含量对神经活动的影响。
影响因子：
18.2
作者：
Zigmond,RE;Bowers,CW
通讯作者：
Bowers,CW