Serum Metabolomics Study Based on LC-MS and Antihypertensive Effect of Uncaria on Spontaneously Hypertensive Rats.
Serum Metabolomics Study Based on LC-MS and Antihypertensive Effect of Uncaria on Spontaneously Hypertensive Rats.
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基于LC-MS的血清代谢组学研究及钩藤对自发性高血压大鼠的降压作用
DOI：
10.1155/2018/9281946
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ju J
中科院分区：
文献类型：
--
作者：
Liu A;Chu YJ;Wang X;Yu R;Jiang H;Li Y;Zhou H;Gong LL;Yang WQ;Ju J
我们前期的研究表明钩藤具有重要的降血压作用，但其在代谢水平上的干预机制尚未完全阐明。因此，本研究采用基于HPLC-TOF/MS的代谢组学和多变量统计分析相结合的方法来探讨钩藤的降压作用机制并评估其降压作用。通过 HPLC-TOF/MS 对血清样品进行分析和鉴定，同时通过偏最小二乘判别分析 (PLS-DA) 和正交偏最小二乘判别分析 (OPLS-DA) 进一步处理获得的数据，以发现扰动的代谢物。获得了不同组之间清晰的聚类，并筛选出7个显着变化的潜在生物标志物。这些生物标志物主要与脂质代谢（二氢神经酰胺、神经酰胺、PC、LysoPC 和 TXA2）以及维生素和氨基酸代谢（烟酰胺核苷、5-HTP）相关。结果表明钩藤可以有效降低血压，部分是通过调节上述生物标志物和代谢途径来实现的。通过对特异性生物标志物的分析验证，进一步了解钩藤的治疗机制和降压作用。代谢组学为中药治疗高血压的疗效评估和潜在机制解析提供了新的视角。
Our previous studies have shown that Uncaria has an important role in lowering blood pressure, but its intervention mechanism has not been clarified completely in the metabolic level. Therefore, in this study, a combination method of HPLC-TOF/MS-based metabolomics and multivariate statistical analyses was employed to explore the mechanism and evaluate the antihypertensive effect of Uncaria. Serum samples were analyzed and identified by HPLC-TOF/MS, while the acquired data was further processed by partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to discover the perturbed metabolites. A clear cluster among the different groups was obtained, and 7 significantly changed potential biomarkers were screened out. These biomarkers were mainly associated with lipid metabolism (dihydroceramide, ceramide, PC, LysoPC, and TXA2) and vitamin and amino acids metabolism (nicotinamide riboside, 5-HTP). The result indicated that Uncaria could decrease the blood pressure effectively, partially by regulating the above biomarkers and metabolic pathways. Analyzing and verifying the specific biomarkers, further understanding of the therapeutic mechanism and antihypertensive effect of Uncaria was acquired. Metabolomics provided a new insight into estimate of the therapeutic effect and dissection of the potential mechanisms of traditional Chinese medicine (TCM) in treating hypertension.
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DOI：
10.2174/1874091x01206010056
发表时间：
2012
期刊：
The open biochemistry journal
影响因子：
--
作者：
Feng L;Manavalan A;Mishra M;Sze SK;Hu JM;Heese K
通讯作者：
Heese K
DOI：
10.1006/bbrc.2002.6614
发表时间：
2002-03-22
影响因子：
3.1
作者：
Cao, ZX;Li, YB
通讯作者：
Li, YB
DOI：
10.1146/annurev.anchem.1.031207.113026
发表时间：
2008-01-01
影响因子：
8
作者：
Lindon, John C.;Nicholson, Jeremy K.
通讯作者：
Nicholson, Jeremy K.
影响因子：
3.5
作者：
Spencer, CGC;Martin, SC;Lip, GYH
通讯作者：
Lip, GYH
影响因子：
1.8
作者：
Nicholson, JK;Lindon, JC;Holmes, E
通讯作者：
Holmes, E