Transcriptional profiling of identified neurons in leech.
Transcriptional profiling of identified neurons in leech.
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DOI：
10.1186/s12864-021-07526-0
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发表时间：
2021-03-25
期刊：
影响因子：
4.4
通讯作者：
Weisblat D
中科院分区：
文献类型：
--
作者：
Heath-Heckman E;Yoo S;Winchell C;Pellegrino M;Angstadt J;Lammardo VB;Bautista D;De-Miguel FF;Weisblat D
长期以来，在神经生物学的模型中，该群体的分子底部在这一组中开始弥合了这一差距，但我们在识别的神经系统中，在这组的神经系统结构和功能上，在这组的神经系统结构和功能上，我们在识别的神经系统的池中进行了rnaseq，虽然是神经系统结构的基础，但在属中，这四种细胞类型是删除。
Bioinformatic analyses identified 3565 putative genes whose expression differed significantly among the samples. These genes clustered into 9 groups which could be associated with one or more of the identified cell types. We verified predicted expression patterns through in situ hybridization on whole CNS ganglia, and found that orthologous genes were for the most part similarly expressed in a divergent leech genus, suggesting evolutionarily constituted roles for these genes.转录分析使我们能够从扩展的基因家族中确定候选表型定义的基因，我们确定了八个高极激活的环核苷酸（HCN）渠道之一ES在T细胞中具有高特异性确定了两个压电基因之一，〜65度/ENAC基因中的两个，以及至少16个瞬时受体电位（TRP）基因之一，是参与感官转导的主要候选物，以参与三种不同类别的水ech机理神经元。
我们的研究定义了水ech中枢神经系统中四种不同神经元类型的不同转录谱，除了从这些数据中提供第二个神经节转录组。
在线版本包含的补充材料可获得10.1186/s12864-021-07526-0。
While leeches in the genus Hirudo have long been models for neurobiology, the molecular underpinnings of nervous system structure and function in this group remain largely unknown. To begin to bridge this gap, we performed RNASeq on pools of identified neurons of the central nervous system (CNS): sensory T (touch), P (pressure) and N (nociception) neurons; neurosecretory Retzius cells; and ganglia from which these four cell types had been removed. Bioinformatic analyses identified 3565 putative genes whose expression differed significantly among the samples. These genes clustered into 9 groups which could be associated with one or more of the identified cell types. We verified predicted expression patterns through in situ hybridization on whole CNS ganglia, and found that orthologous genes were for the most part similarly expressed in a divergent leech genus, suggesting evolutionarily conserved roles for these genes. Transcriptional profiling allowed us to identify candidate phenotype-defining genes from expanded gene families. Thus, we identified one of eight hyperpolarization-activated cyclic-nucleotide gated (HCN) channels as a candidate for mediating the prominent sag current in P neurons, and found that one of five inositol triphosphate receptors (IP3Rs), representing a sub-family of IP3Rs absent from vertebrate genomes, is expressed with high specificity in T cells. We also identified one of two piezo genes, two of ~ 65 deg/enac genes, and one of at least 16 transient receptor potential (trp) genes as prime candidates for involvement in sensory transduction in the three distinct classes of leech mechanosensory neurons. Our study defines distinct transcriptional profiles for four different neuronal types within the leech CNS, in addition to providing a second ganglionic transcriptome for the species. From these data we identified five gene families that may facilitate the sensory capabilities of these neurons, thus laying the basis for future work leveraging the strengths of the leech system to investigate the molecular processes underlying and linking mechanosensation, cell type specification, and behavior. The online version contains supplementary material available at 10.1186/s12864-021-07526-0.
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