Electroacupuncture enhances preproenkephalin mRNA expression in rostral ventrolateral medulla of rats.
Electroacupuncture enhances preproenkephalin mRNA expression in rostral ventrolateral medulla of rats.
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DOI：
10.1016/j.neulet.2010.04.025
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发表时间：
2010-06-21
影响因子：
2.5
通讯作者：
Longhurst JC
中科院分区：
文献类型：
--
作者：
Li M;Tjen-A-Looi SC;Longhurst JC
电针（EA）在麻醉制剂中引起对血压反射升高的长期抑制至少60分钟。因此，EA可以修改交感神经流出和血压升高，通过行动在一些后脑区域，包括头端腹外侧延髓（rVLM）。由于我们以前的数据表明，阿片系统在EA相关的长期抑制前交感神经元的活动在rVLM中发挥作用，我们假设，EA增加前脑啡肽原（PPE）mRNA在这一地区，可能为延长的时间。在α-氯醛糖麻醉下，大鼠在P5-P6穴位（覆盖正中神经）或假手术（无电刺激的针刺位置）接受EA（1-2 mA，2 Hz，0.5 ms）30 min。在接受手术但未接受EA或假手术治疗的对照大鼠中，也评估了rVLM中PPE mRNA的表达。在电针或假处理后20 min、1.5 h或4 h，通过逆转录和定量实时PCR分析rVLM中的PPE mRNA。电针刺激后1.5 h PPE mRNA水平的相对比值（用18 s管家基因标准化）（7.77 ± 1.39，n=6）相对于假手术组（2.84 ± 0.37，n=5）增加，但与假手术组或手术组在相同时间点相比，电针刺激后20 min和4 h均无变化。因此，30分钟的EA短暂刺激脑啡肽的产生在大脑的一个区域，重要地调节交感神经流出，这表明即使是一个单一的短暂的针灸治疗可以增加这种调节性神经肽的表达。
Electroacupuncture (EA) causes prolonged suppression of reflex elevations in blood pressure for at least 60 minutes in anesthetized preparations. Thus, EA can modify sympathetic outflow and elevated blood pressure through actions in a number of hind brain regions, including the rostral ventrolateral medulla (rVLM). Since our previous data show that the opioid system plays a role in EA-related prolonged inhibition of presympathetic neuronal activity in the rVLM, we postulated that EA increases preproenkephalin (PPE) mRNA in this region, possibly for prolonged periods of time. Under α–chloralose anesthesia, rats received EA (1-2 mA, 2 Hz, 0.5 ms) at P5-P6 acupoints (overlying median nerves) or sham (needle placement without electrical stimulation) for 30 min. PPE mRNA in the rVLM also was evaluated in control rats that received surgery but no EA or sham treatment. 20 min, 1.5 h or 4 h following EA or sham treatment, PPE mRNA in the rVLM was analyzed by reverse transcription and quantitative real-time PCR. Relative ratios of PPE mRNA levels (normalized with 18s house keeping gene) were increased 1.5 h after EA stimulation (7.77 ± 1.39, n=6) relative to sham (2.84 ± 0.37, n=5) but were unchanged both 20 min and 4 h after EA, compared to the sham or surgery groups at the same time points. Thus, 30 min of EA transiently stimulates the production of enkephalin in a region of the brain that importantly regulates sympathetic outflow suggesting that even a single brief acupuncture treatment can increase the expression of this modulatory neuropeptide.
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影响因子：
3.3
作者：
Zhou, W;Fu, LW;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
2.9
作者：
Guo, ZL;Moazzami, AR;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
2.5
作者：
Stornetta, RL;Schreihofer, AM;Guyenet, PG
通讯作者：
Guyenet, PG
影响因子：
3.3
作者：
Crisostomo, MM;Li, P;Longhurst, JC
通讯作者：
Longhurst, JC
DOI：
10.1152/ajpheart.1999.276.6.h2127
发表时间：
1999-06-01
影响因子：
4.8
作者：
Chao, DM;Shen, LL;Longhurst, JC
通讯作者：
Longhurst, JC