Freeze-dried somatic cells direct embryonic development after nuclear transfer.
Freeze-dried somatic cells direct embryonic development after nuclear transfer.
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冻干体细胞在核移植后指导胚胎发育。
DOI：
10.1371/journal.pone.0002978
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发表时间：
2008-08-20
期刊：
影响因子：
3.7
通讯作者：
Arav, Amir
中科院分区：
文献类型：
--
作者：
Loi, Pasqualino;Matsukawa, Kazutsugu;Ptak, Grazyna;Clinton, Michael;Fulka, Josef, Jr.;Nathan, Yehudith;Arav, Amir
简单生物体在脱水状态下生存的自然能力早已被人类所利用，冻肉化是长期储存细菌和酵母细胞的首选方法。最近，有人尝试将这一过程应用于血细胞的长期储存。然而，尽管取得了重大进展，但在临床环境中的实际应用仍有一段路要走。相反，到目前为止，还没有报道试图将有核体细胞冻干用于可能的下游应用。在这里，我们证明了在室温下储存3年的冻干体细胞在注射到去核卵母细胞后能够指导胚胎发育。这些显著的结果表明，长期储存细胞系的替代系统现在是可能的，并在生物医学和保护策略领域开辟了前所未有的机会。
The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies.
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影响因子：
46.9
作者：
Wakayama, T;Yanagimachi, R
通讯作者：
Yanagimachi, R
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
46.9
作者：
Eroglu, A;Russo, MJ;Toner, M
通讯作者：
Toner, M
DOI：
10.1080/1024533021000047954
发表时间：
2002-12-01
期刊：
Hematology (Amsterdam, Netherlands)
影响因子：
--
作者：
Fischer, Thomas H;Merricks, Elizabeth P;Read, Marjorie S
通讯作者：
Read, Marjorie S
DOI：
10.1098/rstb.2004.1501
发表时间：
2004-08-29
影响因子：
6.3
作者：
Fuller, W;Forsyth, T;Mahendrasingam, A
通讯作者：
Mahendrasingam, A