Temporal characterization of the functional density of the vasa vasorum by contrast-enhanced ultrasonography maximum intensity projection imaging.
Temporal characterization of the functional density of the vasa vasorum by contrast-enhanced ultrasonography maximum intensity projection imaging.
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DOI：
10.1016/j.jcmg.2010.08.017
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发表时间：
2010-12
影响因子：
14
通讯作者：
Lindner, Jonathan R.
中科院分区：
文献类型：
--
作者：
Lee, Sang Chol;Carr, Chad L.;Davidson, Brian P.;Ellegala, Dilantha;Xie, Aris;Ammi, Azzdine;Belcik, Todd;Lindner, Jonathan R.
我们试图确定对比增强超声（CEU）微血管造影与最大强度投影（MIP）处理是否可以暂时评估血管壁出血模型中血管（VV）的增殖。VV和斑块新血管形成的扩张有助于斑块生长和不稳定性，并且可能由各种刺激（包括血管出血）触发。然而，定量体内方法的VV重塑的时间评估是缺乏的。在24只喂食高脂饲料的家兔中，使用超高频超声引导将自体全血或生理盐水经皮注射到股动脉的中膜-外膜中。注射后1、2和6周，采用MIP处理的CEU成像评估注射部位和对侧对照动脉的功能VV密度。还进行了使用Renathane微管的体外研究，以验证CEU和MIP处理的线性密度测量。体外研究表明，MIP处理的CEU数据反映了相对线性密度的方式，是相对独立的对比剂浓度或微管流速的血管。在使用MIP的CEU上，在血液注射后1周和2周，股动脉VV微血管密度增加了3倍（与对侧对照相比p < 0.01），而盐水注射后VV密度增加最小。6周时，血液处理血管的VV血管密度降低，与盐水注射或对侧对照血管无差异。采用MIP处理的CEU可提供VV功能密度随时间变化的定量数据。该方法可用于评估斑块新生血管的高危特征或对针对斑块新生血管的治疗的反应。
We sought to determine whether contrast-enhanced ultrasound (CEU) microangiography with maximum intensity projection (MIP) processing could temporally evaluate proliferation of the vasa vasorum (VV) in a model of mural hemorrhage. Expansion of the VV and plaque neovascularization contributes to plaque growth and instability and may be triggered by a variety of stimuli, including vascular hemorrhage. However, quantitative in vivo methods for temporal assessment of VV remodeling are lacking. In 24 rabbits fed a high-fat diet, either autologous whole blood or saline was percutaneously injected into the media-adventitia of the femoral artery using ultrahigh-frequency ultrasound guidance. Functional VV density at the injection site and contralateral control artery was assessed 1, 2, and 6 weeks after injection with CEU imaging with MIP processing. In vitro studies with renathane microtubes were also performed to validate linear density measurement with CEU and MIP processing. In vitro studies demonstrated that MIP processing of CEU data reflected the relative linear density of vessels in a manner that was relatively independent of contrast concentration or microtube flow rate. On CEU with MIP, there was a 3-fold increase in femoral artery VV microvascular density at 1 and 2 weeks after blood injection (p < 0.01 vs. contralateral control), whereas VV density increased minimally after saline injection. At 6 weeks, VV vascular density decreased in blood-treated vessels and was not different from saline-injected or contralateral control vessels. CEU with MIP processing can provide quantitative data on temporal changes in the functional density of the VV. This method may be useful for evaluating high-risk features of plaque neovascularization or response to therapies aimed at plaque neovessels.
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DOI：
10.1016/j.echo.2005.10.024
发表时间：
2006-04-01
影响因子：
6.5
作者：
Micari, A;Sklenar, J;Lindner, JR
通讯作者：
Lindner, JR
DOI：
10.1016/s0735-1097(01)01315-8
发表时间：
2001-07-01
影响因子：
24
作者：
Takano, M;Mizuno, K;Sakai, S
通讯作者：
Sakai, S
影响因子：
24
作者：
Coli, Stefano;Magnoni, Marco;Maseri, Attilio
通讯作者：
Maseri, Attilio
影响因子：
8.3
作者：
Vicenzini, Edoardo;Giannoni, Maria Fabrizia;Lenzi, Gian Luigi
通讯作者：
Lenzi, Gian Luigi
DOI：
10.1161/atvbaha.109.196386
发表时间：
2010-01
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Kaufmann BA;Carr CL;Belcik JT;Xie A;Yue Q;Chadderdon S;Caplan ES;Khangura J;Bullens S;Bunting S;Lindner JR
通讯作者：
Lindner JR