Fluorescent Anti-CEA Nanobody for Rapid Tumor-Targeting and Imaging in Mouse Models of Pancreatic Cancer.
Fluorescent Anti-CEA Nanobody for Rapid Tumor-Targeting and Imaging in Mouse Models of Pancreatic Cancer.
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DOI：
10.3390/biom12050711
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发表时间：
2022-05-16
期刊：
影响因子：
5.5
通讯作者：
Bouvet, Michael
中科院分区：
文献类型：
--
作者：
Lwin, Thinzar M.;Turner, Michael A.;Nishino, Hiroto;Amirfakhri, Siamak;Hernot, Sophie;Hoffman, Robert M.;Bouvet, Michael
关键词：
肿瘤特异性靶向与荧光探针可以增强对比，以识别肿瘤手术切除和可视化否则看不见的肿瘤边缘。纳米体是天然存在的最小的抗原结合分子，具有快速的药代动力学。目前的工作证明了与IR800染料结合的荧光抗cea纳米体靶向和标记患者来源的胰腺癌异种移植物的有效性。经静脉给药后，探针在1小时内迅速定位到胰腺癌肿瘤，3小时内肿瘤与背景比为2.0。荧光信号持续时间较长。由于荧光纳米体提供的快速动力学，靶向和成像可以在手术当天进行。
Tumor-specific targeting with fluorescent probes can enhance contrast for identification of cancer during surgical resection and visualize otherwise invisible tumor margins. Nanobodies are the smallest naturally-occurring antigen-binding molecules with rapid pharmacokinetics. The present work demonstrates the efficacy of a fluorescent anti-CEA nanobody conjugated to an IR800 dye to target and label patient derived pancreatic cancer xenografts. After intravenous administration, the probe rapidly localized to the pancreatic cancer tumors within an hour and had a tumor-to-background ratio of 2.0 by 3 h. The fluorescence signal was durable over a prolonged period of time. With the rapid kinetics afforded by fluorescent nanobodies, both targeting and imaging can be performed on the same day as surgery.
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影响因子：
2.5
作者：
Lwin TM;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
影响因子：
3.7
作者：
Lwin TM;Murakami T;Miyake K;Yazaki PJ;Shivley JE;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
影响因子：
3.7
作者：
Hoogstins CES;Boogerd LSF;Sibinga Mulder BG;Mieog JSD;Swijnenburg RJ;van de Velde CJH;Farina Sarasqueta A;Bonsing BA;Framery B;Pèlegrin A;Gutowski M;Cailler F;Burggraaf J;Vahrmeijer AL
通讯作者：
Vahrmeijer AL
DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
--
作者：
Bannas, Peter;Lenz, Alexander;Koch-Nolte, Friedrich
通讯作者：
Koch-Nolte, Friedrich