Long astral microtubules and RACK-1 stabilize polarity domains during maintenance phase in Caenorhabditis elegans embryos.
Long astral microtubules and RACK-1 stabilize polarity domains during maintenance phase in Caenorhabditis elegans embryos.
复制标题
秀丽隐杆线虫胚胎中的维持阶段，长星状微管和RACK-1在维持阶段稳定极性结构域。
DOI：
10.1371/journal.pone.0019020
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发表时间：
2011-04-20
期刊：
影响因子：
3.7
通讯作者：
Skop AR
中科院分区：
文献类型：
--
作者：
Ai E;Poole DS;Skop AR
细胞极性是一个非常保守的过程，对细胞分化、细胞迁移和胚胎发育非常重要。在不同的皮层区域建立后，极性线索必须稳定并保持在流体和动态膜内，以实现适当的细胞不对称性。微管一直被认为是传递细胞所需信号的载体。虽然以前的研究表明，微管在极性的建立中起着关键作用，但在维持阶段对微管的需求仍不清楚。在这项研究中，我们表明，耗尽秀丽隐杆线虫RACK-1，这导致短的星形微管在前中期，具体影响皮质PAR域和发动蛋白本地化的维护。然后，我们研究了在极性维持阶段敲除其他因子的后果，这些因子也会废除星形微管的伸长。我们发现短的星形微管和PAR-6和PAR-2结构域的不稳定性之间的相关性在维持阶段。我们的数据支持星形微管在细胞极性维持阶段的必要作用。
Cell polarity is a very well conserved process important for cell differentiation, cell migration, and embryonic development. After the establishment of distinct cortical domains, polarity cues have to be stabilized and maintained within a fluid and dynamic membrane to achieve proper cell asymmetry. Microtubules have long been thought to deliver the signals required to polarize a cell. While previous studies suggest that microtubules play a key role in the establishment of polarity, the requirement of microtubules during maintenance phase remains unclear. In this study, we show that depletion of Caenorhabditis elegans RACK-1, which leads to short astral microtubules during prometaphase, specifically affects maintenance of cortical PAR domains and Dynamin localization. We then investigated the consequence of knocking down other factors that also abolish astral microtubule elongation during polarity maintenance phase. We found a correlation between short astral microtubules and the instability of PAR-6 and PAR-2 domains during maintenance phase. Our data support a necessary role for astral microtubules in the maintenance phase of cell polarity.
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