Resonance properties of GABAergic interneurons in immature GAD67-GFP mouse neocortex
Resonance properties of GABAergic interneurons in immature GAD67-GFP mouse neocortex
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未成熟 GAD67-GFP 小鼠新皮质中 GABA 能中间神经元的共振特性
DOI：
10.1016/j.brainres.2013.12.032
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发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Luhmann
中科院分区：
文献类型：
--
作者：
Luhmann
阈下共振是不同神经元类别的特征性膜性质，在网络振荡的产生中至关重要，并且将突触输入的整合调谐到特定频率范围。为了研究新皮层GABA能中间神经元是否在出生后早期发育期间已经显示共振行为，我们对出生后（P）P6-P13 GAD 67-GFP敲入小鼠冠状新皮层切片中顶叶区域颗粒上层中视觉识别的中间神经元进行了全细胞膜片钳记录。通过注入频率变化的正弦电流分析了阈下谐振。约50%的GABA能中间神经元出现阈下共振，平均频率为2.0± 0.2Hz（n=38）。膜超极化至-86 mV时，阈下共振的频率和强度减弱。在1 mM Ni 2+的存在下，阈下共振几乎被废除，这表明T-型Ca 2+电流是至关重要的参与共振的产生。相反，阈下共振不受ZD 7288（HCN通道阻断剂）的影响。TTX的应用抑制阈下共振在去极化，但不是超极化的膜电位，表明持续的Na+电流有助于放大的膜共振。总之，这些结果表明，GABA能中间神经元在低频下表达阈下共振，T型Ca 2+和持续Na+电流是产生膜共振的基础。未成熟中间神经元的膜共振可能有助于未成熟新皮层中缓慢振荡活动模式的产生，并增强发育中皮层神经元突触整合的时间精度。
Subthreshold resonance is a characteristic membrane property of different neuronal classes, is critically involved in the generation of network oscillations, and tunes the integration of synaptic inputs to particular frequency ranges. In order to investigate whether neocortical GABAergic interneurons show resonant behavior already during early postnatal development, we performed whole-cell patch-clamp recordings from visually identified interneurons in supragranular layers of parietal regions in coronal neocortical slices from postnatal day (P) P6–P13 GAD67-GFP knock-in mice. Subthreshold resonance was analyzed by injection of sinusoidal current with varying frequency. About 50% of the investigated GABAergic interneurons showed subthreshold resonance with an average frequency of 2.0±0.2 Hz (n=38). Membrane hyperpolarization to –86 mV attenuated the frequency and strength of subthreshold resonance. In the presence of 1 mM Ni2+subthreshold resonance was virtually abolished, suggesting that T-type Ca2+currents are critically involved in the generation of resonance. In contrast, subthreshold resonance was not affected by ZD7288, a blocker of HCN channels. Application of TTX suppressed subthreshold resonance at depolarized, but not hyperpolarized membrane potential, suggesting that persistent Na+current contribute to the amplification of membrane resonance. In summary, these results demonstrate that GABAergic interneurons express subthreshold resonance at low frequencies, with T-type Ca2+and persistent Na+currents underlying the generation of membrane resonance. The membrane resonance of immature interneurons may contribute to the generation of slow oscillatory activity pattern in the immature neocortex and enhance the temporal precision of synaptic integration in developing cortical neurons.
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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DOI：
--
发表时间：
2010
期刊：
Journal of Physiology
影响因子：
--
作者：
Rita Zemankovics;S. Káli;O. Paulsen;T. Freund;N. Hájos
通讯作者：
N. Hájos