Fungal endophytes of aquatic macrophytes: diverse host-generalists characterized by tissue preferences and geographic structure.
Fungal endophytes of aquatic macrophytes: diverse host-generalists characterized by tissue preferences and geographic structure.
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DOI：
10.1007/s00248-013-0324-y
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发表时间：
2014-05
影响因子：
3.6
通讯作者：
Arnold, A. Elizabeth
中科院分区：
文献类型：
--
作者：
Sandberg, Dustin C.;Battista, Lorna J.;Arnold, A. Elizabeth
大多数内生共生体的研究都集中在陆地植物上，忽视了水生生态系统中具有生态和经济重要性的植物。我们评估了美国亚利桑那州北部常见水生植物相关内生真菌的多样性、组成、宿主和组织隶属关系以及地理结构。在两个生长季节从根和光合组织中分离培养内生菌。从 9,600 个植物组织片段中回收了代表 60 个假定物种的总共 226 个分离株。尽管分离频率较低，但内生菌在系统发育上具有多样性且物种丰富。对最彻底采样的物种和水库之间的比较表明，分离频率和多样性在收集时期之间、物种之间、水库之间或作为深度的函数没有显着差异。然而，群落结构在储存库和组织类型之间存在显着差异。对焦点属（青霉属）的系统发育分析证实了对物种边界的估计和知情的群落分析，强调了水生内生菌与沉积物和水生真菌以及邻近陆地植物内生菌的进化枝和基因型级别的关系。这些分析共同提供了对水生植物根部和叶子内生关联的首次定量检查，可用于优化调查策略，以有效捕获当地和区域尺度的真菌生物多样性。
Most studies of endophytic symbionts have focused on terrestrial plants, neglecting the ecologically and economically important plants present in aquatic ecosystems. We evaluated the diversity, composition, host- and tissue affiliations, and geographic structure of fungal endophytes associated with common aquatic plants in northern Arizona, USA. Endophytes were isolated in culture from roots and photosynthetic tissues during two growing seasons. A total of 226 isolates representing 60 putative species was recovered from 9,600 plant tissue segments. Although isolation frequency was low, endophytes were phylogenetically diverse and species-rich. Comparisons among the most thoroughly sampled species and reservoirs revealed that isolation frequency and diversity did not differ significantly between collection periods, among species, among reservoirs, or as a function of depth. However, community structure differed significantly among reservoirs and tissue types. Phylogenetic analyses of a focal genus (Penicillium) corroborated estimates of species boundaries and informed community analyses, highlighting clade- and genotype-level affiliations of aquatic endophytes with both sediment- and waterborne fungi, and endophytes of proximate terrestrial plants. Together these analyses provide a first quantitative examination of endophytic associations in roots and foliage of aquatic plants and can be used to optimize survey strategies for efficiently capturing fungal biodiversity at local and regional scales.
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影响因子：
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通讯作者：
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