Reduction pathway of graphene oxide affects conjugation-mediated horizontal gene transfer under environmental conditions
Reduction pathway of graphene oxide affects conjugation-mediated horizontal gene transfer under environmental conditions
复制标题
氧化石墨烯的还原途径影响环境条件下缀合介导的水平基因转移
DOI：
10.1016/j.cej.2022.138301
复制
发表时间：
2022
影响因子：
15.1
通讯作者：
Xiang Xiao
中科院分区：
文献类型：
--
作者：
Qiurong Zhang;Xinlei Liu;Huixian Zhou;Yilin Lu;Yangyang Fan;Lijun Wu;Xiang Xiao
• Effect of the reduction pathway of GO on ARGs dissemination was investigated. • GO underwent environmental reduction when exposed to illumination and Fe 2+ . • Graphene radicals generated by light-reduced GO exacerbated ARGs transfer. • Physical wrapping of Fe 2+ -reduced GO aggregates weakened ARGs dissemination. Antimicrobial resistance in the environment has become a critical issue and its dissemination mediated by nanomaterials is receiving increasing attention. However, current studies overlook the contribution of the environmental transformation of nanomaterials to the horizontal transfer of antibiotic resistance genes (ARGs). Herein, graphene oxide (GO) was transformed by simulated illumination or ferrous iron (Fe 2+ ) to mimic the naturally occurring GO reduction, and for the first time, the distinct effects of reduced GO (rGO) on ARGs horizontal transfer were observed. Compared with pristine GO, the transfer of plasmid-borne ARGs was dramatically enhanced by light-reduced GO. The remarkable production of stabilized graphene radical excited by ultraviolet irradiation triggered bacterial oxidative stress and contributed to membrane permeability. However, when reduced by Fe 2+ , the facilitation of ARGs transfer mediated by GO was attenuated, which was attributed to the physical wrapping of large rGO aggregates that hindered plasmid transfer between bacteria. Furthermore, the generated transconjugant strain via GO-mediated conjunction showed stronger macrophage infection than the original Escherichia coli under antibiotic treatment. These findings emphasize that nanomaterial-mediated ARGs transfer depends on its transformation pathways in a realistic environment, which is helpful for the understanding of the dissemination of antimicrobial resistance.
登录
查看更多内容
影响因子：
8.8
作者：
Guo, Mei-Ting;Zhang, Guo-Sheng
通讯作者：
Zhang, Guo-Sheng
还原氧化石墨烯的还原途径依赖性细胞毒性
DOI：
10.1039/c8en00242h
发表时间：
2018-06
期刊：
Environmental Science: Nano
影响因子：
--
作者：
Qiurong Zhang;Xiaolei Liu;Hongyan Meng;Sijin Liu;Chengdong Zhang
通讯作者：
Chengdong Zhang
影响因子：
10
作者：
Lammel T;Boisseaux P;Fernández-Cruz ML;Navas JM
通讯作者：
Navas JM
影响因子：
16.6
作者：
Schnaider L;Brahmachari S;Schmidt NW;Mensa B;Shaham-Niv S;Bychenko D;Adler-Abramovich L;Shimon LJW;Kolusheva S;DeGrado WF;Gazit E
通讯作者：
Gazit E
影响因子：
11.4
作者：
Goodwin DG Jr;Adeleye AS;Sung L;Ho KT;Burgess RM;Petersen EJ
通讯作者：
Petersen EJ