Laryngeal Reconstruction Using Tissue-Engineered Implants in Pigs: A Pilot Study.
Laryngeal Reconstruction Using Tissue-Engineered Implants in Pigs: A Pilot Study.
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DOI：
10.1002/lary.29282
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Halum S
中科院分区：
文献类型：
--
作者：
Brookes S;Zhang L;Puls TJ;Kincaid J;Voytik-Harbin S;Halum S
目前还没有治疗方法可以恢复半喉切除术后的动态喉功能。我们已经证明，在大鼠半喉切除术后，动态功能可以恢复。在这里，我们首次报告了这类概念研究的证明，即这种先前发表的技术可扩展到猪模型。动物研究。对三只尤卡坦迷你猪进行了肌肉和脂肪活检。分离肌肉祖细胞（MPCs）和脂肪干细胞（ASCs），培养3周。迷你猪接受了左偏侧部分喉切除术，保留了左杓状软骨并横断了喉返神经。每层用组织工程植入物替代：1)由致密I型寡聚胶原组成的脱细胞粘膜层，2)由自体MPCs和定向寡聚胶原分化诱导表达运动终板（MEE）组成的骨骼肌层，以及3)由自体ASCs和致密寡聚胶原分化为软骨组成的软骨层。在术后2周和4周以及8周研究终点监测愈合情况。动物表现出适当的体重增加，无误吸事件，发声。视像喉镜检查显示在第4周出现血管形成和再上皮化的进展性愈合。组织学上，植入物无免疫反应，与宿主组织完全融合，神经和血管长入。这项初步研究首次采用定制的自体干细胞衍生的多层组织工程植入物进行了跨壁垂直喉部切除术并成功修复。N/A
There are currently no treatments available that restore dynamic laryngeal function after hemilaryngectomy. We have shown that dynamic function can be restored post hemilaryngectomy in a rat model. Here, we report in a first of its kind, proof of concept study that this previously published technique is scalable to a porcine model. Animal study. Muscle and fat biopsies were taken from three Yucatan minipigs. Muscle progenitor cells (MPCs) and adipose stem cells (ASCs) were isolated and cultured for 3 weeks. The minipigs underwent a left laterovertical partial laryngectomy sparing the left arytenoid cartilage and transecting the recurrent laryngeal nerve. Each layer was replaced with a tissue-engineered implant: 1) an acellular mucosal layer composed of densified Type I oligomeric collagen, 2) a skeletal muscle layer composed of autologous MPCs and aligned oligomeric collagen differentiated and induced to express motor endplates (MEE), and 3) a cartilage layer composed of autologous ASCs and densified oligomeric collagen differentiated to cartilage. Healing was monitored at 2 and 4 weeks post-op, and at the 8 week study endpoint. Animals demonstrated appropriate weight gain, no aspiration events, and audible phonation. Video laryngoscopy showed progressive healing with vascularization and re-epithelialization present at 4 weeks. On histology, there was no immune reaction to the implants and there was complete integration into host tissue with nerve and vascular ingrowth. This pilot study represents a first in which a transmural vertical partial laryngectomy was performed and successfully repaired with a customized, autologous stem cell-derived multi-layered tissue-engineered implant. N/A
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影响因子：
1.9
作者：
BLAKESLEE, DB;BANKS, RE;BROOKS, D
通讯作者：
BROOKS, D
影响因子：
6.2
作者：
Gorti, GK;Birchall, MA;Bailey, M
通讯作者：
Bailey, M
影响因子：
2.6
作者：
Halum, Stacey L.;McRae, Bryan;Bijangi-Vishehsaraei, Khadijeh;Hiatt, Kelly
通讯作者：
Hiatt, Kelly
影响因子：
6
作者：
Ansari, Tahera;Lange, Peggy;Birchall, Martin A.
通讯作者：
Birchall, Martin A.
影响因子：
2.6
作者：
Zhang, Hongji;Voytik-Harbin, Sherry;Halum, Stacey
通讯作者：
Halum, Stacey