Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
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DOI：
10.3109/14653241003649528
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发表时间：
2010-07
期刊：
影响因子：
4.5
通讯作者：
Gimble JM
中科院分区：
文献类型：
--
作者：
Yu G;Wu X;Dietrich MA;Polk P;Scott LK;Ptitsyn AA;Gimble JM
Adipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yield from human subcutaneous lipoaspirates. This study reports our recent experience isolating and immunophenotypically characterizing ASCs from >60 human subjects of mean age 43.6 and mean body mass index of 27. We examined the ASC yield per unit volume of lipoaspirate tissue, their surface antigen profile based on flow cytometry, their histochemical differentiation potential along the adipogenic and osteogenic pathways, and their expression of adipogenic mRNAs by transcriptomic microarray and RT-PCR. The population (n = 64) of predominantly Caucasian (84.3%) female (90.6%) donors had a mean age of 43.6 ± 11.1 years and a mean body mass index of 27.0 ± 3.8. A yield of 375 ± 142 × 103 ASC was obtained per ml of lipoaspirate within a 4.1 ± 0.7 day culture period (n = 62). The ASC population was uniformly CD29+ CD34+CD44loCD45loCD73+CD90+CD105+ and capable of undergoing both adipogenesis and osteogenesis in vitro based on Oil Red O and Alizarin Red staining, respectively. Adipogenic differentiation was associated with the significant induction of multiple mRNAs associated with lipid storage and synthesis based microarray analysis of n = 3 donors. During an adipogenic differentiation time course, representative mRNAs (adiponectin, C/EBPα, leptin, LPL) displayed increases of several orders of magnitude. These findings demonstrate the reproducibility of subcutaneous lipoaspirates as a consistent and abundant source of functional ASCs from donors across a spectrum of ages and BMIs. These results have relevance to regenerative medical applications exploiting autologous or allogeneic ASCs for soft and hard tissue engineering.
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影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
5.2
作者：
McIntosh, Kevin;Zvonic, Sanjin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
37.8
作者：
Miranville, A;Heeschen, C;Bouloumié, A
通讯作者：
Bouloumié, A
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影响因子：
2.8
作者：
Lopez, Mandi J.;McIntosh, Kevin R.;Spencer, Nakia D.;Borneman, Jade N.;Horswell, Ronald;Anderson, Paul;Yu, Gang;Gaschen, Lorrie;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
4.2
作者：
Justesen, J;Stenderup, K;Kassem, M
通讯作者：
Kassem, M