Nanoscale membrane curvature sorts lipid phases and alters lipid diffusion
Nanoscale membrane curvature sorts lipid phases and alters lipid diffusion
复制标题
纳米级膜曲率对脂质相进行分类并改变脂质扩散
DOI：
10.1016/j.bpj.2023.01.001
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发表时间：
2023
影响因子：
3.4
通讯作者：
Kelly, Christopher V.
中科院分区：
文献类型：
--
作者：
Woodward, Xinxin;Javanainen, Matti;Fábián, Balázs;Kelly, Christopher V.
纳米尺度膜形状和组成的精确时空控制是个体和集体分子行为复杂相互作用的结果。在这里，我们使用单分子定位显微镜和计算模拟来观察单脂质在不同成分、相、温度和曲率的模型膜中的扩散和分选。在半径为50纳米的纳米颗粒上形成了支持的脂质双层，以模拟自然发生的膜芽的大小，如内吞坑和病毒包膜的形成。弯曲膜吸收了液体紊乱的脂相，同时改变了示踪脂质的扩散和分选。只有当嵌入能够在低温下维持脂相分离的膜中时，偏爱无序的荧光脂质才会在纳米尺度曲率上进行分类并经历更快的扩散。当样品温度高于平面膜的混相温度时，曲率诱导的分选和更快的扩散也发生了，这表明纳米尺度的曲率可以在均匀的膜中诱导相分离。进一步证实和理解这些结果提供了连续和粗粒度的分子动力学模拟，分别具有显式和自发曲率-相位耦合。曲率诱导的膜组成不均一性和动力学改变只有在曲率与脂相分离的耦合下才能实现。这些交叉验证的结果表明，脂质相、分子扩散和纳米级膜曲率的复杂相互作用对膜功能至关重要。
The precise spatiotemporal control of nanoscale membrane shape and composition is the result of a complex interplay of individual and collective molecular behaviors. Here, we employed single-molecule localization microscopy and computational simulations to observe single-lipid diffusion and sorting in model membranes with varying compositions, phases, temperatures, and curvatures. Supported lipid bilayers were created over 50-nm-radius nanoparticles to mimic the size of naturally occurring membrane buds, such as endocytic pits and the formation of viral envelopes. The curved membranes recruited liquid-disordered lipid phases while altering the diffusion and sorting of tracer lipids. Disorder-preferring fluorescent lipids sorted to and experienced faster diffusion on the nanoscale curvature only when embedded in a membrane capable of sustaining lipid phase separation at low temperatures. The curvature-induced sorting and faster diffusion even occurred when the sample temperature was above the miscibility temperature of the planar membrane, implying that the nanoscale curvature could induce phase separation in otherwise homogeneous membranes. Further confirmation and understanding of these results are provided by continuum and coarse-grained molecular dynamics simulations with explicit and spontaneous curvature-phase coupling, respectively. The curvature-induced membrane compositional heterogeneity and altered dynamics were achieved only with a coupling of the curvature with a lipid phase separation. These cross-validating results demonstrate the complex interplay of lipid phases, molecular diffusion, and nanoscale membrane curvature that are critical for membrane functionality.
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由电子束制造的基底施加的脂质域像素化图案。
DOI：
10.1021/la3008415
发表时间：
2012
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Ogunyankin,MariaO;Torres,Andrea;Yaghmaie,Frank;Longo,MarjorieL
通讯作者：
Longo,MarjorieL
与曲率耦合的脂质双层中的筏形成
影响因子：
3.4
作者：
S. Sadeghi;M. Müller;R. Vink
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影响因子：
4
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DOI：
10.1073/pnas.0907354106
发表时间：
2009-12-29
影响因子：
11.1
作者：
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DOI：
10.1101/2020.05.28.121830
发表时间：
2020-05
期刊：
bioRxiv
影响因子：
--
作者：
X. Woodward;C. Kelly
通讯作者：
X. Woodward;C. Kelly