Lipid domain pixelation patterns imposed by e-beam fabricated substrates.

Lipid domain pixelation patterns imposed by e-beam fabricated substrates.
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由电子束制造的基底施加的脂质域像素化图案。

DOI:
10.1021/la3008415
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发表时间:
2012
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
通讯作者:
Longo,MarjorieL
Longo,MarjorieL
中科院分区:
--
文献类型:
--
作者:
Ogunyankin,MariaO;Torres,Andrea;Yaghmaie,Frank;Longo,MarjorieL

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这项工作描述了一种形成纳米级像素化类脂结构域的技术,这些结构域自组织成位于正方形晶格上的几何图案。在这一过程中,将脂质多双层堆叠沉积在由电子束光刻形成的带有方形点阵阵列的二氧化硅衬底上,凸块的侧面为半球形。域图案被显示为被限制在凸起之间的平面栅格中,并且由连接的和单独的域像素组成。对不同尺寸晶格的分析表明,磁畴图案的形成是由机械能最小化和堆积约束驱动的。我们展示了单一晶格大小和晶格大小从微米到100纳米尺度变化的梯度,适用于生物分子的精确排列、图案化和传输,这些生物分子划分到类脂域。
This work describes a technique for forming nanometer-scale pixilated lipid domains that are self-organized into geometric patterns residing on a square lattice. In this process, a lipid multibilayer stack is deposited onto a silica substrate patterned with a square lattice array of bumps, hemispherical on their sides, formed by electron beam lithography. Domain patterns are shown to be confined to the flat grid between the bumps and composed of connected and individual domain pixels. Analysis of lattices of varying sizes shows that domain pattern formation is driven by mechanical energy minimization and packing constraints. We demonstrate single lattice sizes and a gradient in lattice size varying from the micrometer to the 100 nm scale applicable to precise arraying, patterning, and transport of biomolecules that partition to lipid domains.
通过选择性囊泡吸附和纳米形貌破裂实现流体膜的非破坏性微图案化。
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