Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model.
Distinguishing synaptic vesicle precursor navigation of microtubule ends with a single rate constant model.
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DOI：
10.1038/s41598-021-82836-7
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发表时间：
2021-02-09
影响因子：
4.6
通讯作者：
Yogev S
中科院分区：
文献类型：
--
作者：
Gramlich MW;Balseiro-Gómez S;Tabei SMA;Parkes M;Yogev S
轴突马达驱动的货物利用微管细胞骨架，以引导货物，如突触囊泡前体（SVP），到需要它们的地方。这种运输需要囊泡在微米的距离内移动。最近观察到，有限的微管长度可以作为抑制SVP并增加运输所需时间的障碍。SVP到达微管的末端并暂停，直到它们可以导航到相邻的微管以继续运输。轴突SVP通过微管末端导航以继续移动的机制尚不清楚。在这篇文章中，我们模拟实验观察到的小泡暂停在微管末端的C。优雅的我们发现，一个单一的速率常数模型再现的时间SVP暂停在MT结束。该模型基于SVP必须从其当前微管分离并重新附着到相邻微管的时间。我们发现，囊泡停顿时间是不同的顺行和逆行运动，这表明囊泡利用不同的蛋白质在正负端网站。最后，我们表明，囊泡不可能利用拔河一样的机制和反向，以浏览微管末端。
Axonal motor driven cargo utilizes the microtubule cytoskeleton in order to direct cargo, such as synaptic vesicle precursors (SVP), to where they are needed. This transport requires vesicles to travel up to microns in distance. It has recently been observed that finite microtubule lengths can act as roadblocks inhibiting SVP and increasing the time required for transport. SVPs reach the end of a microtubule and pause until they can navigate to a neighboring microtubule in order to continue transport. The mechanism(s) by which axonal SVPs navigate the end of a microtubule in order to continue mobility is unknown. In this manuscript we model experimentally observed vesicle pausing at microtubule ends in C. elegans. We show that a single rate-constant model reproduces the time SVPs pause at MT-ends. This model is based on the time an SVP must detach from its current microtubule and re-attach to a neighboring microtubule. We show that vesicle pause times are different for anterograde and retrograde motion, suggesting that vesicles utilize different proteins at plus and minus end sites. Last, we show that vesicles do not likely utilize a tug-of-war like mechanism and reverse direction in order to navigate microtubule ends.
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影响因子：
16.2
作者：
Klassen, Matthew P.;Wu, Ye E.;Maeder, Celine I.;Nakae, Isei;Cueva, Juan G.;Lehrman, Emily K.;Tada, Minoru;Gengyo-Ando, Keiko;Wang, George J.;Goodman, Miriam;Mitani, Shohei;Kontani, Kenji;Katada, Toshiaki;Shen, Kang
通讯作者：
Shen, Kang
DOI：
10.1126/science.1254198
发表时间：
2014-07-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
McKenney RJ;Huynh W;Tanenbaum ME;Bhabha G;Vale RD
通讯作者：
Vale RD
影响因子：
2.5
作者：
Che DL;Chowdary PD;Cui B
通讯作者：
Cui B
影响因子：
56.9
作者：
Kural, C;Kim, H;Selvin, PR
通讯作者：
Selvin, PR
DOI：
10.1073/pnas.91.15.6865
发表时间：
1994-07-19
影响因子：
11.1
作者：
HACKNEY, DD
通讯作者：
HACKNEY, DD