A time course transcriptome analysis of brains from sex-undifferentiated Nile tilapia discloses genes associated with high-temperature-induced masculinization
A time course transcriptome analysis of brains from sex-undifferentiated Nile tilapia discloses genes associated with high-temperature-induced masculinization
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对性别未分化尼罗罗非鱼大脑的时间进程转录组分析揭示了与高温诱导的雄性化相关的基因
DOI：
10.1016/j.aquaculture.2020.735762
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发表时间：
2021-01
期刊：
影响因子：
4.5
通讯作者：
Xiang Shan Ji
中科院分区：
文献类型：
--
作者：
Zhi Lei Yao;Hongju Chen;Yan Zhao;Zhenjie Cao;Hui Wang;Xiang Shan Ji
鱼类性别决定机制可分为基因型 (GSD)、温度 (TSD) 或基因型加温度 (GSD + TE) 效应。热敏感期（TSP）期间的水温可能会影响各种硬骨鱼物种性腺或大脑中许多基因的表达水平。然而，目前尚未对尼罗罗非鱼大脑在TSP期间高温开始至结束期间基因表达变化的研究进行研究。在本研究中，我们对 5 dah（孵化后天）尼罗罗非鱼的大脑样本进行了 RNA 测序，这些尼罗罗非鱼接受了 6 小时（5 dah +6 h）和 24 小时（5 dah +24 h）的高温处理。结果显示，在 5 dah +6 h 和 5 dah +24 h 时，雌性对照 (FC) 与高温处理雌性 (FT) 比较组中存在 307 个和 933 个差异表达基因 (DEG)，FC 与雄性对照 (MC) 比较组中分别存在 606 个和 762 个 DEG。为了验证RNA-Seq获得的DEG，随机选择10个基因进行qRT-PCR。结果显示，10个基因中各基因在两个时间点的RNA-Seq和qRT-PCR结果之间的表达水平相似，并且RNA-Seq和qRT-PCR数据之间存在很强的相关性（r=0.532，P<.01）。利用本实验室前期5 dah +12 d的尼罗罗非鱼脑转录组数据，对3个时间点（5 dah +6 h、5 dah +24 h和5 dah +12 d）的尼罗罗非鱼脑转录组数据进行分析表明，Kdm6bb等与性别分化和高温处理相关的6个基因显着上调，而细胞色素P450等13个基因显着上调。 2J6 在 FC 与 FT 对照组中在两个或三个检查时间点显着下调。同时，热休克蛋白家族中的 4 个基因（Dnaja、Dnajb1a、热休克蛋白 70 和热休克 70 kDa 蛋白 1）在 2 个检测时间点高温处理后表达量显着上调，Hspa8b 在 3 个检测时间点高温处理后表达量显着上调。最后，选择Kdm6bb进行原位杂交进一步验证。这项研究发现了许多与高温诱导的雄性化相关的基因，并为尼罗罗非鱼以及其他具有 GSD + TE 效应的物种的分子机制提供了新的见解。
Fish sex-determining mechanisms can be classified as genotypic (GSD), temperature (TSD), or genotypic plus temperature (GSD + TE) effects. Water temperature during thermosensitive periods (TSPs) could affect the expression levels of many genes in the gonad or the brain in various teleost species. However, studies on gene expression changes in the brain of Nile tilipia from the start to the end of high-temperature during TSP have not been investigated. In this study, we performed RNA-Seq on brain samples from 5 dah (days after hatching) Nile tilapia that underwent high temperature treatment for 6 h (5 dah +6 h) and 24 h (5 dah +24 h). The results showed that 307 and 933 differential expressed genes (DEGs) were present in the female controls (FC) vs high-temperature-treated females (FT) comparison group and that 606 and 762 DEGs were in the FC vs male controls (MC) comparison group at 5 dah +6 h and 5 dah +24 h, respectively. To validate the DEGs obtained by RNA-Seq, 10 genes were randomly selected to perform qRT-PCR. The results showed that the expression levels of each gene among the 10 genes were similar between the RNA-Seq and qRT-PCR results at the two time points and that there was a strong correlation between the RNA-Seq and qRT-PCR data (r= 0.532,P< .01). Using previous Nile tilapia brain transcriptome data at 5 dah +12 d from our lab, profiling analysis of the Nile tilapia brain transcriptome data at three time points (5 dah +6 h, 5 dah +24 h and 5 dah +12 d) indicated that six genes associated with sex differentiation and high-temperature treatment, includingKdm6bb, were significantly upregulated, while 13 genes, including cytochromeP450 2J6, were significantly downregulated in the FC vs FT comparison group at two or three examined time points. Meanwhile, the expression levels of four genes (Dnaja,Dnajb1a, heat shock protein 70, and heat shock 70 kDa protein 1) in heat shock protein family were significantly upregulated after high-temperature treatment at two examined time points, andHspa8bwere significantly upregulated after high-temperature treatment at three examined time points. Finally, theKdm6bbwas selected for further verification using in situ hybridization. This study found many genes associated with high-temperature induced masculinization and provided new insights into the molecular mechanisms in Nile tilapia as well as in other species with GSD + TE effects.
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影响因子：
4.5
作者：
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通讯作者：
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影响因子：
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RNA-Seq 分析揭示罗非鱼性别决定和分化过程中的转录组显示
影响因子：
4.4
作者：
Tao W;Chen J;Tan D;Yang J;Sun L;Wei J;Conte MA;Kocher TD;Wang D
通讯作者：
Wang D