Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex.
Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex.
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DOI：
10.1038/s41467-023-37844-8
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发表时间：
2023-04-24
影响因子：
16.6
通讯作者：
Anastassiou, Costas A.
中科院分区：
文献类型：
--
作者：
Wei, Yina;Nandi, Anirban;Jia, Xiaoxuan;Siegle, Joshua H.;Denman, Daniel;Lee, Soo Yeun;Buchin, Anatoly;Van Geit, Werner;Mosher, Clayton P.;Olsen, Shawn;Anastassiou, Costas A.
大脑由许多细胞类别组成，但体内电生理学记录通常无法识别和监测它们在行为动物中的活动。在这里，我们采用了一种系统的方法，通过计算建模和光标记实验，将来自实验的细胞，多模态体外特性与体内记录的单位联系起来。我们在小鼠视觉皮层中发现了两个单通道和六个多通道簇，它们在活动、皮层深度和行为方面具有不同的体内特性。我们使用生物物理模型来映射两个一个和六个多通道集群到特定的体外类，具有独特的形态，兴奋性和电导特性，解释其独特的细胞外签名和功能特性。这些概念进行了测试，在地面真实光标记实验与两个抑制类揭示不同的体内特性。这种多模态方法提供了一种强大的方法来分离体内簇并从第一原理推断它们的细胞特性。理解不同神经元细胞类型的功能作用是具有挑战性的。在这里，作者将多模态体外细胞特性与小鼠视觉皮层的体内生理学联系起来。
The brain consists of many cell classes yet in vivo electrophysiology recordings are typically unable to identify and monitor their activity in the behaving animal. Here, we employed a systematic approach to link cellular, multi-modal in vitro properties from experiments with in vivo recorded units via computational modeling and optotagging experiments. We found two one-channel and six multi-channel clusters in mouse visual cortex with distinct in vivo properties in terms of activity, cortical depth, and behavior. We used biophysical models to map the two one- and the six multi-channel clusters to specific in vitro classes with unique morphology, excitability and conductance properties that explain their distinct extracellular signatures and functional characteristics. These concepts were tested in ground-truth optotagging experiments with two inhibitory classes unveiling distinct in vivo properties. This multi-modal approach presents a powerful way to separate in vivo clusters and infer their cellular properties from first principles. Understanding functional role of different neuronal cell types is challenging. Here the authors associate multi-modal in vitro cell properties with in vivo physiology of mouse visual cortex.
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影响因子：
16.2
作者：
Chen G;Zhang Y;Li X;Zhao X;Ye Q;Lin Y;Tao HW;Rasch MJ;Zhang X
通讯作者：
Zhang X
影响因子：
9.8
作者：
Harris KD;Hochgerner H;Skene NG;Magno L;Katona L;Bengtsson Gonzales C;Somogyi P;Kessaris N;Linnarsson S;Hjerling-Leffler J
通讯作者：
Hjerling-Leffler J
细胞外田地和电流的起源-EEG，ECOG，LFP和尖峰。
DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Buzsáki G;Anastassiou CA;Koch C
通讯作者：
Koch C
影响因子：
25
作者：
Gouwens NW;Sorensen SA;Berg J;Lee C;Jarsky T;Ting J;Sunkin SM;Feng D;Anastassiou CA;Barkan E;Bickley K;Blesie N;Braun T;Brouner K;Budzillo A;Caldejon S;Casper T;Castelli D;Chong P;Crichton K;Cuhaciyan C;Daigle TL;Dalley R;Dee N;Desta T;Ding SL;Dingman S;Doperalski A;Dotson N;Egdorf T;Fisher M;de Frates RA;Garren E;Garwood M;Gary A;Gaudreault N;Godfrey K;Gorham M;Gu H;Habel C;Hadley K;Harrington J;Harris JA;Henry A;Hill D;Josephsen S;Kebede S;Kim L;Kroll M;Lee B;Lemon T;Link KE;Liu X;Long B;Mann R;McGraw M;Mihalas S;Mukora A;Murphy GJ;Ng L;Ngo K;Nguyen TN;Nicovich PR;Oldre A;Park D;Parry S;Perkins J;Potekhina L;Reid D;Robertson M;Sandman D;Schroedter M;Slaughterbeck C;Soler-Llavina G;Sulc J;Szafer A;Tasic B;Taskin N;Teeter C;Thatra N;Tung H;Wakeman W;Williams G;Young R;Zhou Z;Farrell C;Peng H;Hawrylycz MJ;Lein E;Ng L;Arkhipov A;Bernard A;Phillips JW;Zeng H;Koch C
通讯作者：
Koch C
影响因子：
64.5
作者：
Gouwens NW;Sorensen SA;Baftizadeh F;Budzillo A;Lee BR;Jarsky T;Alfiler L;Baker K;Barkan E;Berry K;Bertagnolli D;Bickley K;Bomben J;Braun T;Brouner K;Casper T;Crichton K;Daigle TL;Dalley R;de Frates RA;Dee N;Desta T;Lee SD;Dotson N;Egdorf T;Ellingwood L;Enstrom R;Esposito L;Farrell C;Feng D;Fong O;Gala R;Gamlin C;Gary A;Glandon A;Goldy J;Gorham M;Graybuck L;Gu H;Hadley K;Hawrylycz MJ;Henry AM;Hill D;Hupp M;Kebede S;Kim TK;Kim L;Kroll M;Lee C;Link KE;Mallory M;Mann R;Maxwell M;McGraw M;McMillen D;Mukora A;Ng L;Ng L;Ngo K;Nicovich PR;Oldre A;Park D;Peng H;Penn O;Pham T;Pom A;Popović Z;Potekhina L;Rajanbabu R;Ransford S;Reid D;Rimorin C;Robertson M;Ronellenfitch K;Ruiz A;Sandman D;Smith K;Sulc J;Sunkin SM;Szafer A;Tieu M;Torkelson A;Trinh J;Tung H;Wakeman W;Ward K;Williams G;Zhou Z;Ting JT;Arkhipov A;Sümbül U;Lein ES;Koch C;Yao Z;Tasic B;Berg J;Murphy GJ;Zeng H
通讯作者：
Zeng H