Classification of electrophysiological and morphological neuron types in the mouse visual cortex.
Classification of electrophysiological and morphological neuron types in the mouse visual cortex.
复制标题
DOI：
10.1038/s41593-019-0417-0
复制
发表时间：
2019-07
影响因子：
25
通讯作者：
Koch C
中科院分区：
文献类型：
--
作者：
Gouwens NW;Sorensen SA;Berg J;Lee C;Jarsky T;Ting J;Sunkin SM;Feng D;Anastassiou CA;Barkan E;Bickley K;Blesie N;Braun T;Brouner K;Budzillo A;Caldejon S;Casper T;Castelli D;Chong P;Crichton K;Cuhaciyan C;Daigle TL;Dalley R;Dee N;Desta T;Ding SL;Dingman S;Doperalski A;Dotson N;Egdorf T;Fisher M;de Frates RA;Garren E;Garwood M;Gary A;Gaudreault N;Godfrey K;Gorham M;Gu H;Habel C;Hadley K;Harrington J;Harris JA;Henry A;Hill D;Josephsen S;Kebede S;Kim L;Kroll M;Lee B;Lemon T;Link KE;Liu X;Long B;Mann R;McGraw M;Mihalas S;Mukora A;Murphy GJ;Ng L;Ngo K;Nguyen TN;Nicovich PR;Oldre A;Park D;Parry S;Perkins J;Potekhina L;Reid D;Robertson M;Sandman D;Schroedter M;Slaughterbeck C;Soler-Llavina G;Sulc J;Szafer A;Tasic B;Taskin N;Teeter C;Thatra N;Tung H;Wakeman W;Williams G;Young R;Zhou Z;Farrell C;Peng H;Hawrylycz MJ;Lein E;Ng L;Arkhipov A;Bernard A;Phillips JW;Zeng H;Koch C
了解大脑中细胞类型的多样性一直是一个持久的挑战，需要在多个维度上详细描述单个神经元。为了系统地分析哺乳动物神经元的形态电特性，我们使用脑切片中的标准化膜片钳记录和基于生物胞素的神经元重建建立了单细胞表征管道。我们建立了一个公众可访问的在线数据库，艾伦细胞类型数据库，以显示这些数据集。从来自成年实验室小鼠视觉皮层的1，938个神经元测量了内在生理特性，从461个重建神经元测量了形态学特性，并且452个神经元具有两种测量结果。定量特征用于使用无监督方法将神经元分类为不同类型。我们建立了一个分类的形态学和电生理学定义的细胞类型的皮质与17个E型，38个M型，46个ME型，并发现良好的对应关系与以前定义的转录组细胞类型和子类使用相同的转基因小鼠品系。
Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To systematically profile morpho-electric properties of mammalian neurons, we established a single cell characterization pipeline using standardized patch clamp recordings in brain slices and biocytin-based neuronal reconstructions. We built a publicly-accessible online database, the Allen Cell Types Database, to display these data sets. Intrinsic physiological properties were measured from 1,938 neurons from the adult laboratory mouse visual cortex, morphological properties were measured from 461 reconstructed neurons, and 452 neurons had both measurements available. Quantitative features were used to classify neurons into distinct types using unsupervised methods. We established a taxonomy of morphologically- and electrophysiologically-defined cell types for this region of cortex with 17 e-types, 38 m-types, and 46 me-types and found good correspondence with previously-defined transcriptomic cell types and subclasses using the same transgenic mouse lines.
登录
查看更多内容
影响因子：
2.5
作者：
Hattox, Alexis M.;Nelson, Sacha B.
通讯作者：
Nelson, Sacha B.
影响因子：
3.7
作者：
Druckmann, Shaul;Hill, Sean;Segev, Idan
通讯作者：
Segev, Idan
影响因子：
16.2
作者：
Kim EJ;Juavinett AL;Kyubwa EM;Jacobs MW;Callaway EM
通讯作者：
Callaway EM
通过三重免疫染色鉴定小鼠视觉皮层中 GABA 能神经元的多种不同亚型。
影响因子：
2.9
作者：
Gonchar, Yuri;Wang, Quanxin;Burkhalter, Andreas
通讯作者：
Burkhalter, Andreas
DOI：
10.1126/science.aab3415
发表时间：
2015-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dehorter N;Ciceri G;Bartolini G;Lim L;del Pino I;Marín O
通讯作者：
Marín O