The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer.
The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer.
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DOI：
10.1186/1471-2180-10-101
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发表时间：
2010-04-06
期刊：
影响因子：
4.2
通讯作者：
Choi Y
中科院分区：
文献类型：
--
作者：
Chun J;Kim KY;Lee JH;Choi Y
虽然小鼠长期以来一直作为牙周炎的动物模型，但有关其本土口腔微生物群组成的信息有限。本研究的目的是通过使用最新型号的焦磷酸测序仪Roche/454基因组测序仪FLX Titanium进行广泛的平行焦磷酸测序来表征小鼠口腔细菌植物群。此外，还评估了Toll样受体（TLR）2缺乏对口腔微生物群的影响。野生型（n = 4）和TLR 2敲除（n = 4）C57 BL/6小鼠的8个口腔细菌群落通过分析通过焦磷酸测序获得的16 S rRNA基因的80，046个读段来表征。排除PCR引物，每个测序产物的平均长度为443 bp。小鼠口腔细菌群落的平均物种丰富度估计约为200种，但群落仅由两个主要门和几个物种主导。因此，细菌群落相对简单。小鼠口腔微生物群的细菌组成与人类的显著不同，并且缺乏TLR 2对小鼠口腔微生物群的影响可以忽略不计。使用Roche/454 FLX Titanium的焦磷酸测序成功表征了小鼠口腔细菌群落。相对简单的小鼠口腔细菌群落不受TLR 2缺乏的影响。这些发现将为进一步研究牙周病原体在牙周炎小鼠模型中的作用提供基础。
Although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. The aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a Roche/454 Genome Sequencer FLX Titanium. In addition, the effect of Toll-like receptor (TLR) 2 deficiency on oral microbiota was evaluated. Eight oral bacterial communities of wild-type (n = 4) and TLR2 knock-out (n = 4) C57BL/6 mice were characterized by analyzing 80,046 reads of 16S rRNA genes obtained by pyrosequencing. Excluding the PCR primers, the average length of each sequencing product was 443 bp. The average species richness of the murine oral bacterial communities was estimated to be about 200, but the communities were dominated by only two main phyla and several species. Therefore, the bacterial communities were relatively simple. The bacterial composition of the murine oral microbiota was significantly different from that of humans, and the lack of TLR2 had a negligible effect on the murine oral microbiota. Pyrosequencing using the Roche/454 FLX Titanium successfully characterized mouse oral bacterial communities. The relatively simple oral bacterial communities of mice were not affected by TLR2 deficiency. These findings will provide a basis for future studies on the role of periodontal pathogens in the murine model of periodontitis.
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影响因子：
--
作者：
Marcotte, H;Lavoie, MC
通讯作者：
Lavoie, MC
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
3
作者：
BAKER, PJ;EVANS, RT;ROOPENIAN, DC
通讯作者：
ROOPENIAN, DC
影响因子：
--
作者：
MARCOTTE, H;RODRIGUE, L;LAVOIE, MC
通讯作者：
LAVOIE, MC
影响因子：
4.4
作者：
Burns, Elia;Bachrach, Gilad;Nussbaum, Gabriel
通讯作者：
Nussbaum, Gabriel