Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs.
Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs.
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DOI：
10.1093/nar/gku618
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发表时间：
2014-08
影响因子：
14.9
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Kimura S;Miyauchi K;Ikeuchi Y;Thiaville PC;Crécy-Lagard Vd;Suzuki T
甲基化是一种涉及合成和修饰生物活性分子的多用途反应，包括rna。n6 -甲基苏氨酸氨基甲酰腺苷（m6t6A）是在细菌、昆虫、植物和哺乳动物的trna的37位发现的转录后修饰。在这里，我们报道了在大肠杆菌中，yaeB（重命名为trmO）编码tRNA甲基转移酶，负责tRNAThr中m6t6A的n6 -甲基，特异性为ACY密码子。TrmO具有独特的单片β桶结构，不属于任何已知的甲基转移酶类别。重组TrmO采用s -腺苷- l-蛋氨酸（AdoMet）作为甲基供体，在tRNAThr中甲基化t6A，形成m6t6A。因此，TrmO/YaeB代表了一类新的adomet依赖性甲基转移酶（Class VIII）。在ΔtrmO菌株中，m6t6A被转化为环状t6A (ct6A)，这表明t6A是m6t6A和ct6A的共同前体。此外，t6A的n6甲基化增强了thr操纵子的衰减活性，表明TrmO确保了ACY的有效解码。我们还发现了一个人类同源基因TRMO，这表明m6t6A在从细菌到哺乳动物的生物体的解码微调中起着普遍的作用。
Methylation is a versatile reaction involved in the synthesis and modification of biologically active molecules, including RNAs. N6-methyl-threonylcarbamoyl adenosine (m6t6A) is a post-transcriptional modification found at position 37 of tRNAs from bacteria, insect, plants, and mammals. Here, we report that in Escherichia coli, yaeB (renamed as trmO) encodes a tRNA methyltransferase responsible for the N6-methyl group of m6t6A in tRNAThr specific for ACY codons. TrmO has a unique single-sheeted β-barrel structure and does not belong to any known classes of methyltransferases. Recombinant TrmO employs S-adenosyl-L-methionine (AdoMet) as a methyl donor to methylate t6A to form m6t6A in tRNAThr. Therefore, TrmO/YaeB represents a novel category of AdoMet-dependent methyltransferase (Class VIII). In a ΔtrmO strain, m6t6A was converted to cyclic t6A (ct6A), suggesting that t6A is a common precursor for both m6t6A and ct6A. Furthermore, N6-methylation of t6A enhanced the attenuation activity of the thr operon, suggesting that TrmO ensures efficient decoding of ACY. We also identified a human homolog, TRMO, indicating that m6t6A plays a general role in fine-tuning of decoding in organisms from bacteria to mammals.
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影响因子：
11.4
作者：
Ikeuchi, Yoshiho;Kitahara, Kei;Suzuki, Tsutomu
通讯作者：
Suzuki, Tsutomu
影响因子：
14.9
作者：
Kaminska KH;Purta E;Hansen LH;Bujnicki JM;Vester B;Long KS
通讯作者：
Long KS
影响因子：
14.9
作者：
Jühling F;Mörl M;Hartmann RK;Sprinzl M;Stadler PF;Pütz J
通讯作者：
Pütz J
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
14.9
作者：
Daugeron MC;Lenstra TL;Frizzarin M;El Yacoubi B;Liu X;Baudin-Baillieu A;Lijnzaad P;Decourty L;Saveanu C;Jacquier A;Holstege FC;de Crécy-Lagard V;van Tilbeurgh H;Libri D
通讯作者：
Libri D