Advantages of Molecular Weight Identification during Native MS Screening.
Advantages of Molecular Weight Identification during Native MS Screening.
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DOI：
10.1055/a-0608-4870
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发表时间：
2018-11
期刊：
影响因子：
2.7
通讯作者：
Quinn R
中科院分区：
文献类型：
--
作者：
Khan A;Bresnick A;Cahill S;Girvin M;Almo S;Quinn R
配体-蛋白质复合物的天然质谱检测允许快速检测来自提取物和级分的载脂蛋白和钙结合的S100 A4（金属结合蛋白S100家族的成员）、T细胞/跨膜、免疫球蛋白（IG）和粘蛋白蛋白3以及具有IG和ITIM（基于免疫受体酪氨酸的抑制基序）结构域的T细胞免疫受体的天然产物结合剂前体蛋白。基于分子量，检测到与所有四种蛋白质结合的常见命中。其中有7种常见的化合物被鉴定为芹菜素6-C-β-D-葡萄糖苷、8-C-α-L-阿拉伯糖苷、野慈姑苷、4′，5-二羟基-7-甲氧基黄烷酮-6-C-芸香糖苷、马钱苷酸、6-C-葡萄糖基柚皮素、鹰嘴豆芽素A 7-O-芸香糖苷和槲皮素3-O-芸香糖苷。命中的质量引导分离和NMR鉴定证实了配体-蛋白质MS复合物中配体的质量准确度。因此，通过电喷雾电离傅立叶变换质谱法从配体-蛋白质复合物的分子量ID允许快速去复制。使用电喷雾电离傅立叶变换质谱的天然质谱是用于去复制和代谢组学分析的工具。
Native mass spectrometry detection of ligand-protein complexes allowed rapid detection of natural product binders of apo and calcium-bound S100A4 (a member of the metal binding protein S100 family), T cell/transmembrane, immunoglobulin (Ig), and mucin protein 3, and T cell immunoreceptor with Ig and ITIM (immunoreceptor tyrosine-based inhibitory motif) domains precursor protein from extracts and fractions. Based on molecular weight common hits were detected binding to all four proteins. Seven common hits were identified as apigenin 6-C-β-D-glucoside 8-C-α-L-arabinoside, sweroside, 4′,5-dihydroxy-7-methoxyflavanone-6-C-rutinoside, loganin acid, 6-C-glucosylnaringenin, biochanin A 7-O-rutinoside and quercetin 3-O-rutinoside. Mass guided isolation and NMR identification of hits confirmed the mass accuracy of the ligand in the ligand-protein MS complexes. Thus, molecular weight ID from ligand-protein complexes by electrospray ionization Fourier transform mass spectrometry allowed rapid dereplication. Native mass spectrometry using electrospray ionization Fourier transform mass spectrometry is a tool for dereplication and metabolomics analysis.
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影响因子：
2.9
作者：
Camp, David;Campitelli, Marc;Quinn, Ronald J.
通讯作者：
Quinn, Ronald J.
影响因子：
5.1
作者：
Kang, TH;Jeong, SJ;Kim, YC
通讯作者：
Kim, YC
影响因子：
30.5
作者：
Chiba, Shigeki;Baghdadi, Muhammad;Akiba, Hisaya;Yoshiyama, Hironori;Kinoshita, Ichiro;Dosaka-Akita, Hirotoshi;Fujioka, Yoichiro;Ohba, Yusuke;Gorman, Jacob V.;Colgan, John D.;Hirashima, Mitsuomi;Uede, Toshimitsu;Takaoka, Akinori;Yagita, Hideo;Jinushi, Masahisa
通讯作者：
Jinushi, Masahisa
影响因子：
--
作者：
Bai, Jianwen;Li, Xiaoyan;Li, Qinchuan
通讯作者：
Li, Qinchuan
影响因子：
4.6
作者：
Hammami, S;Ben Jannet, H;Mighri, Z
通讯作者：
Mighri, Z