Ablation of glucosinolate accumulation in the oil crop Camelina sativa by targeted mutagenesis of genes encoding the transporters GTR1 and GTR2 and regulators of biosynthesis MYB28 and MYB29.
Ablation of glucosinolate accumulation in the oil crop Camelina sativa by targeted mutagenesis of genes encoding the transporters GTR1 and GTR2 and regulators of biosynthesis MYB28 and MYB29.
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DOI：
10.1111/pbi.13936
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发表时间：
2023-01
影响因子：
13.8
通讯作者：
Doermann, Peter
中科院分区：
文献类型：
--
作者：
Hoelzl, Georg;Rezaeva, Barno Ruzimurodovna;Kumlehn, Jochen;Doermann, Peter
亚麻荠是一种投入成本低、抗非生物和生物胁迫的油料作物。芥子油苷的存在，植物代谢产物与不利的健康影响，限制了使用亚麻荠作为人类和动物的营养。基于Cas9核酸内切酶的硫代葡萄糖转运蛋白CsGTR1和CsGTR2的三种同源物的靶向诱变引起硫代葡萄糖含量的强烈降低，突出了这种方法在六倍体作物中灭活多个基因的能力。每个转录因子CsMYB 28和CsMYB 29的三个同源物的突变导致硫代葡萄糖苷的完全损失，代表了第一个不含硫代葡萄糖苷的油菜科作物。油和蛋白质含量和脂肪酸组成的csgtr1，csgtr2和csmyb28，csmyb29突变体种子没有受到影响。芥子油苷的减少和消除提高了亚麻荠油和压榨饼的质量，从而符合调节人类消费和动物饲养的芥子油苷水平的国际标准。
Camelina sativa is an oil crop with low input costs and resistance to abiotic and biotic stresses. The presence of glucosinolates, plant metabolites with adverse health effects, restricts the use of camelina for human and animal nutrition. Cas9 endonuclease‐based targeted mutagenesis of the three homeologs of each of the glucosinolate transporters CsGTR1 and CsGTR2 caused a strong decrease in glucosinolate amounts, highlighting the power of this approach for inactivating multiple genes in a hexaploid crop. Mutagenesis of the three homeologs of each of the transcription factors CsMYB28 and CsMYB29 resulted in the complete loss of glucosinolates, representing the first glucosinolate‐free Brassicaceae crop. The oil and protein contents and the fatty acid composition of the csgtr1csgtr2 and csmyb28csmyb29 mutant seeds were not affected. The decrease and elimination of glucosinolates improves the quality of the oil and press cake of camelina, which thus complies with international standards regulating glucosinolate levels for human consumption and animal feeding.
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影响因子：
7.4
作者：
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通讯作者：
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