Unconditional and conditional QTL analyses of seed fatty acid composition in Brassica napus L.
Unconditional and conditional QTL analyses of seed fatty acid composition in Brassica napus L.
复制标题
甘蓝型油菜种子脂肪酸组成的非条件和条件QTL分析。
DOI：
10.1186/s12870-018-1268-7
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发表时间：
2018-03-23
影响因子：
5.3
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen F;Zhang W;Yu K;Sun L;Gao J;Zhou X;Peng Q;Fu S;Hu M;Long W;Pu H;Chen S;Wang X;Zhang J
B的脂肪酸组成。油菜籽决定了油的营养价值和工业价值，并影响种子发芽。许多研究报道了基于表型数据的C16：0、C18：0、C18：1、C18：2和C18：3之间的相关性;然而，B中脂肪酸组成的遗传基础。人们对napus的认识还不很透彻。在这项研究中，无条件和有条件的数量试验位点（QTL）定位分析进行了重组自交系在六个环境。非条件定位共检测到C16：0、C18：0和C18：2共21个QTL，C18：1共16个，C18：3共22个。通过Meta分析，将置信区间重叠的QTL整合到71个多效性独特QTL中。除C18：0外，在大多数环境中，两个主效QTL uuqA 5 -6和uuqA 5 -7同时影响脂肪酸，同源基因脂肪酸去饱和酶2（FAD 2）和甘油-3-磷酸sn-2-酰基转移酶5（GPAT 5）位于uuqA 5 -6的置信区间内，磷脂酸磷酸水解酶1（PAH 1）位于uuqA 5 -7的置信区间内。通过条件作图分析，在C16：0、C18：0、C18：1、C18：2和C18：3上分别检测到49、30、48、60和45个共有QTL。总共有128个独特的QTL随后从232个条件共有QTL整合。比较分析表明，两种作图方法共定位到63个QTL，另外65个QTL仅在条件作图中定位到。因此，条件QTL定位脂肪酸可能会发现许多其他的QTL，抑制了其他性状的影响。这些结果为在QTL水平上更好地理解脂肪酸之间的遗传关系提供了有用的信息。本文的在线版本（10.1186/s12870-018-1268-7）包含补充材料，可供授权用户使用。
The fatty acid composition of B. napus’ seeds determines the oil’s nutritional and industrial values, and affects seed germination. Many studies have reported correlations among C16:0, C18:0, C18:1, C18:2 and C18:3 based on phenotypic data; however, the genetic basis of the fatty acid composition in B. napus is still not well understood. In this study, unconditional and conditional quantitative trail locus (QTL) mapping analyses were conducted using a recombinant inbred line in six environments. In total, 21 consensus QTLs each for C16:0, C18:0 and C18:2, 16 for C18:1 and 22 for C18:3 were detected by unconditional mapping. The QTLs with overlapping confidence intervals were integrated into 71 pleiotropically unique QTLs by meta-analysis. Two major QTLs, uuqA5–6 and uuqA5–7, simultaneously affected the fatty acids, except C18:0, in most of environments, with the homologous genes fatty acid desaturase 2 (FAD2) and glycerol-3-phosphate sn-2-acyltransferase 5 (GPAT5) occurring in the confidence interval of uuqA5–6, while phosphatidic acid phosphohydrolase 1 (PAH1) was assigned to uuqA5–7. Moreover, 49, 30, 48, 60 and 45 consensus QTLs were detected for C16:0, C18:0, C18:1, C18:2 and C18:3, respectively, by the conditional mapping analysis. In total, 128 unique QTLs were subsequently integrated from the 232 conditional consensus QTLs. A comparative analysis revealed that 63 unique QTLs could be identified by both mapping methodologies, and 65 additional unique QTLs were only identified in conditional mapping. Thus, conditional QTL mapping for fatty acids may uncover numerous additional QTLs that were inhibited by the effects of other traits. These findings provide useful information for better understanding the genetic relationships among fatty acids at the QTL level. The online version of this article (10.1186/s12870-018-1268-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1199/tab.0133
发表时间：
2010-01-01
期刊：
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影响因子：
--
作者：
Li-Beisson, Yonghua;Shorrosh, Basil;Ohlrogge, John
通讯作者：
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DOI：
10.1073/pnas.1318511111
发表时间：
2014-01-21
影响因子：
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影响因子：
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