Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
复制标题
通过扫描激光光学断层扫描（SLOT）对耳蜗内组织进行三维成像
DOI：
10.1117/12.2211066
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ripken T
中科院分区：
文献类型：
--
作者：
Tinne N;Nolte L;Antonopoulos GC;Schulze J;Andrade J;Heisterkamp A;Meyer H;Warnecke A;Majdani O;Ripken T
本研究的重点是应用扫描激光光学断层扫描技术（SLOT）对人耳蜗体内解剖结构进行无损可视化。SLOT是一种基于激光的高效显微技术，它允许对透明大型标本（高达1 cm3）的内部结构进行层析成像。因此，在耳科领域，这种技术最方便内耳解剖的体外研究。为此目的，成像前的准备工作包括机械辅助脱钙、脱水以及耳蜗样品的光学清除。在这里，我们展示了利用吸收和自身荧光作为对比机制，在微米范围内以光学分辨率显示人耳蜗硬组织和软组织结构的结果。此外，我们还将结果与仅基于吸收的临床金标准x射线微断层扫描（micro- ct, μCT）方法进行了比较。总的来说，SLOT技术的优势在于涵盖了其他光学显微镜技术（如荧光或散射）中已知的所有对比机制。由于这个原因，抗体染色方案已经开发出来，它还可以选择性地绘制耳蜗内的细胞结构。因此，我们提出了啮齿动物耳蜗槽成像的结果，显示特定的解剖结构，如毛细胞和神经丝通过荧光。综上所述，本研究表明，SLOT是耳科领域对内耳微观结构进行实时可视化的理想工具。
The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochleaex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for anex vivostudy of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure.
影响因子：
3.7
作者：
Eickhoff R;Lorbeer RA;Scheiblich H;Heisterkamp A;Meyer H;Stern M;Bicker G
通讯作者：
Bicker G
影响因子：
3.8
作者：
Lorbeer, Raoul-Amadeus;Heidrich, Marko;Heisterkamp, Alexander
通讯作者：
Heisterkamp, Alexander
影响因子：
1.4
作者：
Postnov, A;Zarowski, A;Peeters, S
通讯作者：
Peeters, S