STED nanoscopy with fluorescent quantum dots.
STED nanoscopy with fluorescent quantum dots.
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DOI：
10.1038/ncomms8127
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发表时间：
2015-05-18
影响因子：
16.6
通讯作者：
Hell, Stefan W.
中科院分区：
文献类型：
--
作者：
Hanne, Janina;Falk, Henning J.;Goerlitz, Frederik;Hoyer, Patrick;Engelhardt, Johann;Sahl, Steffen J.;Hell, Stefan W.
到目前为止，广泛流行的量子点分子标签不能用作step的标准荧光问题（刺激发射消耗）纳米镜检查。量子点实际上是通过越来越流行的775 nm steed激光来实现的。 um-dot闪烁的标签在很大程度上被兴奋的组合作用和st型梁的组合抑制了。
Sted纳米镜检查可以在这里具有广泛的荧光问题，作者表明，在蜂窝情况下，可以用作为生物标记的stem来超级分辨出明亮且非常稳定的荧光量子点。
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discovery that STED nanoscopy of several red-emitting commercially available quantum dots is in fact successfully realized by the increasingly popular 775 nm STED laser light. A resolution of presently ∼50 nm is demonstrated for single quantum dots, and sub-diffraction resolution is further shown for imaging of quantum-dot-labelled vimentin filaments in fibroblasts. The high quantum-dot photostability enables repeated STED recordings with >1,000 frames. In addition, we have evidence that the tendency of quantum-dot labels to blink is largely suppressed by combined action of excitation and STED beams. Quantum-dot STED significantly expands the realm of application of STED nanoscopy, and, given the high stability of these probes, holds promise for extended time-lapse imaging. STED nanoscopy enables sub-diffraction imaging with a wide range of fluorescent probes. Here, the authors show that a bright and very photostable class of fluorescent quantum dots can be super-resolved with STED as biolabels in cellular contexts.
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影响因子：
16.6
作者：
Irvine, Scott E.;Staudt, Thorsten;Hell, Stefan W.
通讯作者：
Hell, Stefan W.
DOI：
10.1007/978-1-60761-404-3_11
发表时间：
2010-01-01
期刊：
LIVE CELL IMAGING: METHODS AND PROTOCOLS
影响因子：
--
作者：
Wurm, Christian A.;Neumann, Daniel;Jakobs, Stefan
通讯作者：
Jakobs, Stefan
影响因子：
3.8
作者：
Bingen, Pit;Reuss, Matthias;Hell, Stefan W.
通讯作者：
Hell, Stefan W.
影响因子：
1.8
作者：
Rittweger, E.;Wildanger, D.;Hell, S. W.
通讯作者：
Hell, S. W.
影响因子：
3.7
作者：
Arnspang, Eva C.;Brewer, Jonathan R.;Lagerholm, B. Christoffer
通讯作者：
Lagerholm, B. Christoffer