Differential effects of leucine supplementation in young and aged mice at the onset of skeletal muscle regeneration.
Differential effects of leucine supplementation in young and aged mice at the onset of skeletal muscle regeneration.
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DOI：
10.1016/j.mad.2016.05.007
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发表时间：
2016-07
影响因子：
5.3
通讯作者：
Washington, Tyrone A.
中科院分区：
文献类型：
--
作者：
Perry, Richard A., Jr.;Brown, Lemuel A.;Lee, David E.;Brown, Jacob L.;Baum, Jamie I.;Greene, Nicholas P.;Washington, Tyrone A.
衰老会降低骨骼肌对损伤的反应能力。亮氨酸已被证明可以靶向骨骼肌中的蛋白质合成途径，从而增强这种反应。然而，在骨骼肌再生开始时，衰老对亮氨酸诱导的蛋白质合成改变的影响尚未完全阐明。本研究的目的是确定衰老是否会改变骨骼肌再生和亮氨酸诱导的蛋白质合成标记物的改变。将幼龄（3月龄）和老年（24月龄）雌性C57BL/6J小鼠胫骨前肌注射布比卡因或PBS，并给予小鼠随意补充亮氨酸或正常饮用水。注射后3 d分别分析蛋白质合成和降解标志物的蛋白和基因表达。幼鼠损伤后，补充亮氨酸仅能提高p-p70S6K。在老年小鼠中，亮氨酸显示出损伤前后p-mTOR含量升高，损伤后p-4EBP-1含量升高。此外，在老年小鼠中，亮氨酸显示出损伤后较高的相对p70S6K含量。我们的研究表明，补充亮氨酸对年轻和老年小鼠骨骼肌再生开始时蛋白质合成的影响是不同的。
Aging decreases the ability of skeletal muscle to respond to injury. Leucine has been demonstrated to target protein synthetic pathways in skeletal muscle thereby enhancing this response. However, the effect of aging on leucine-induced alterations in protein synthesis at the onset of skeletal muscle regeneration has not been fully elucidated. The purpose of this study was to determine if aging alters skeletal muscle regeneration and leucine-induced alterations in markers of protein synthesis. The tibialis anterior of young (3 months) and aged (24 months) female C57BL/6J mice were injected with either bupivacaine or PBS, and the mice were given ad libitum access to leucine-supplemented or normal drinking water. Protein and gene expression of markers of protein synthesis and degradation, respectively, were analyzed at three days post-injection. Following injury in young mice, leucine supplementation was observed to elevate only p-p70S6K. In aged mice, leucine was shown to elicit higher p-mTOR content with and without injury, and p-4EBP-1 content post-injury. Additionally in aged mice, leucine was shown to elicit higher content of relative p70S6K post-injury. Our study shows that leucine supplementation affects markers of protein synthesis at the onset of skeletal muscle regeneration differentially in young and aged mice.
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影响因子：
3.7
作者：
Macotela Y;Emanuelli B;Bång AM;Espinoza DO;Boucher J;Beebe K;Gall W;Kahn CR
通讯作者：
Kahn CR
影响因子：
4.2
作者：
Anthony, JC;Anthony, TG;Jefferson, LS
通讯作者：
Jefferson, LS
影响因子：
3.5
作者：
Costa Junior, Jose M.;Rosa, Morgana R.;Carneiro, Everardo M.
通讯作者：
Carneiro, Everardo M.
DOI：
10.1111/j.1532-5415.1997.tb00986.x
发表时间：
1997-01-01
影响因子：
6.3
作者：
Fried, LP;Guralnik, JM
通讯作者：
Guralnik, JM
影响因子：
6.3
作者：
Brown, L. A.;Lee, D. E.;Washington, T. A.
通讯作者：
Washington, T. A.