Combining Multiple Assays Improves Detection and Serotyping of Foot-and-Mouth Disease Virus. A Practical Example with Field Samples from East Africa.
Combining Multiple Assays Improves Detection and Serotyping of Foot-and-Mouth Disease Virus. A Practical Example with Field Samples from East Africa.
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DOI：
10.3390/v13081583
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发表时间：
2021-08-10
期刊：
影响因子：
--
通讯作者：
Pezzoni G
中科院分区：
文献类型：
--
作者：
Foglia EA;Lembo T;Kazwala R;Ekwem D;Shirima G;Grazioli S;Brocchi E;Pezzoni G
口蹄疫病毒(FMDV)的多种血清型和地区型在流行地区流行，对当地造成相当大的影响。此外，对新领域的介绍也是非常令人关注的。事实上，近年来，世界各地都记录到由从原来地区逃逸的地形型病毒引起的多次口蹄疫疫情。在这两种情况下，快速和准确的诊断，包括确定引起特定暴发的血清型和拓扑型，在实施最有效和最适当的措施以控制疾病传播方面发挥着重要作用。在本研究中，我们描述了2012-2018年间在坦桑尼亚北部(东非，EA)收集的一组水泡样本上一系列口蹄疫诊断和分型工具的性能。具体地说，我们用针对3D序列的实时RT-PCR法检测了这些样本，检测了口蹄疫病毒基于单抗的抗原(Ag)检测和血清分型ELISA试剂盒，在LFBKαVβ6细胞上进行了病毒分离(VI)，以及一组由四个拓扑型特异性实时RT-PCR组成的小组，专门为EA的循环菌株量身定做。3D实时RT-PCR诊断灵敏度最高，但缺乏分型能力。71%的标本匀浆检测到口蹄疫病毒，而VI结合银酶联免疫吸附试验检测口蹄疫病毒的效率为82%。在93%的样本中，拓扑特异性实时RT-PCRS鉴定和分型口蹄疫病毒。然而，SAT1实时荧光RT-PCR的失败率最高(20%)。简而言之，拓扑特异性实时RT-PCR对EA FMDV具有最高的血清分型能力，尽管需要进行四次检测，而敏感度较低的Ag-ELISA是最方便的，因此适用于任何实验室水平。综上所述，当四种对比试验联合使用时，诊断和血清分型性能都接近100%。
Multiple serotypes and topotypes of foot-and-mouth disease virus (FMDV) circulate in endemic areas, posing considerable impacts locally. In addition, introductions into new areas are of great concern. Indeed, in recent years, multiple FMDV outbreaks, caused by topotypes that have escaped from their original areas, have been recorded in various parts of the world. In both cases, rapid and accurate diagnosis, including the identification of the serotype and topotype causing the given outbreaks, plays an important role in the implementation of the most effective and appropriate measures to control the spread of the disease. In the present study, we describe the performance of a range of diagnostic and typing tools for FMDV on a panel of vesicular samples collected in northern Tanzania (East Africa, EA) during 2012–2018. Specifically, we tested these samples with a real-time RT-PCR targeting 3D sequence for pan-FMDV detection; an FMDV monoclonal antibody-based antigen (Ag) detection and serotyping ELISA kit; virus isolation (VI) on LFBKαVβ6 cell line; and a panel of four topotype-specific real-time RT-PCRs, specifically tailored for circulating strains in EA. The 3D real-time RT-PCR showed the highest diagnostic sensitivity, but it lacked typing capacity. Ag-ELISA detected and typed FMDV in 71% of sample homogenates, while VI combined with Ag-ELISA for typing showed an efficiency of 82%. The panel of topotype-specific real-time RT-PCRs identified and typed FMDV in 93% of samples. However, the SAT1 real-time RT-PCR had the highest (20%) failure rate. Briefly, topotype-specific real-time RT-PCRs had the highest serotyping capacity for EA FMDVs, although four assays were required, while the Ag-ELISA, which was less sensitive, was the most user-friendly, hence suitable for any laboratory level. In conclusion, when the four compared tests were used in combination, both the diagnostic and serotyping performances approached 100%.
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影响因子：
1.5
作者：
Reid, Scott M.;Ebert, Katja;King, Donald P.
通讯作者：
King, Donald P.
影响因子：
3.1
作者：
Bachanek-Bankowska, Katarzyna;Mero, Herieth R.;King, Donald P.
通讯作者：
King, Donald P.
影响因子：
9.4
作者：
LaRocco, Michael;Krug, Peter W.;Rodriguez, Luis L.
通讯作者：
Rodriguez, Luis L.
DOI：
10.2460/javma.2002.220.1636
发表时间：
2002-06-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Callahan, JD;Brown, F;Nelson, WM
通讯作者：
Nelson, WM
DOI：
10.20506/rst.35.3.2565
发表时间：
2016-12-01
影响因子：
0.6
作者：
Knowles, N. J.;Wadsworth, J.;King, D. P.
通讯作者：
King, D. P.