OsHIPL1, a hedgehog-interacting protein-like 1 protein, increases seed vigour in rice.
OsHIPL1, a hedgehog-interacting protein-like 1 protein, increases seed vigour in rice.
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OsHIPL1是一种与刺猬相互作用的蛋白质样1，可提高水稻种子活力。
DOI：
10.1111/pbi.13812
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发表时间：
2022-07
影响因子：
13.8
通讯作者：
Cheng, Jinping
中科院分区：
文献类型：
--
作者：
He, Ying;Chen, Shanshan;Liu, Kexin;Chen, Yongji;Cheng, Yanhao;Zeng, Peng;Zhu, Peiwen;Xie, Ting;Chen, Sunlu;Zhang, Hongsheng;Cheng, Jinping
培育高活力水稻品种是水稻直播栽培的关键，而种子活力调控的分子基础至今仍不清楚。本研究克隆了一个新基因OsHIPL 1，该基因编码水稻种子活力主效QTL qSV 3的一个致病基因hedgehog相互作用蛋白样1蛋白。OsHIPL 1主要定位于细胞膜和细胞核。RNA测序（RNA-seq）结果表明，阿坝相关基因参与OsHIPL 1对水稻种子活力的调控。与IR 26植物相比，在OsHIPLl突变体和NIL-qsv 3系的萌发种子中测量到更高水平的内源阿坝，具有两个上调的阿坝生物合成基因（OsZEP和OsNCED 4）和一个下调的阿坝催化剂基因OsABA 8 ox 3。脱落酸不敏感基因OsABI 3、OsABI 4和OsABI 5在OsHIPL 1突变体和NIL-qsv 3株系的萌发种子中的表达较IR 26植株显著上调。这些结果表明，OsHIPL 1基因可能通过调节水稻种子萌发过程中内源阿坝水平和OsABIs的表达来调节种子活力。同时，我们发现OsHIPL 1与水通道蛋白OsPIP 1;1相互作用，影响水稻种子的吸水能力，从而促进水稻种子萌发。基于对水稻种质单核苷酸多态性数据的分析，我们鉴定了OsHIPL 1的Hap 1单倍型，其与种子萌发正相关。本研究为OsHIPL 1基因影响种子活力的分子机制提供了新的思路。
The cultivation of rice varieties with high seed vigour is vital for the direct seeding of rice, and the molecular basis of regulation of seed vigour remains elusive. Here, we cloned a new gene OsHIPL1, which encodes hedgehog‐interacting protein‐like 1 protein as a causal gene of the major QTL qSV3 for rice seed vigour. OsHIPL1 was mainly localized in the plasma membrane and nucleus. RNA sequencing (RNA‐seq) revealed that the ABA‐related genes were involved in the OsHIPL1 regulation of seed vigour in rice. The higher levels of endogenous ABA were measured in germinating seeds of OsHIPL1 mutants and NIL‐qsv3 line compared to IR26 plants, with two up‐regulated ABA biosynthesis genes (OsZEP and OsNCED4) and one down‐regulated ABA catabolism gene OsABA8ox3. The expression of abscisic acid‐insensitive 3 (OsABI3), OsABI4 and OsABI5 was significantly up‐regulated in germinating seeds of OsHIPL1 mutants and NIL‐qsv3 line compared to IR26 plants. These results indicate that the regulation of seed vigour of OsHIPL1 may be through modulating endogenous ABA levels and altering OsABIs expression during seed germination in rice. Meanwhile, we found that OsHIPL1 interacted with the aquaporin OsPIP1;1, then affected water uptake to promote rice seed germination. Based on analysis of single‐nucleotide polymorphism data of rice accessions, we identified a Hap1 haplotype of OsHIPL1 that was positively correlated with seed germination. Our findings showed novel insights into the molecular mechanism of OsHIPL1 on seed vigour.
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影响因子：
7.4
作者：
Debeaujon, I;Koornneef, M
通讯作者：
Koornneef, M
DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
44.1
作者：
Guo, Lei;Wang, Zi Yi;Gu, Hongya
通讯作者：
Gu, Hongya
影响因子：
0.8
作者：
Eizenga, Georgia C.;Ali, Md Liakat;McCouch, Susan R.
通讯作者：
McCouch, Susan R.
影响因子：
5.4
作者：
Fujino, K;Sekiguchi, H;Yano, M
通讯作者：
Yano, M