Maize DNA Methylation in Response to Drought Stress Is Involved in Target Gene Expression and Alternative Splicing.
Maize DNA Methylation in Response to Drought Stress Is Involved in Target Gene Expression and Alternative Splicing.
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响应干旱胁迫的玉米 DNA 甲基化涉及靶基因表达和选择性剪接
DOI：
10.3390/ijms22158285
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发表时间：
2021-07-31
影响因子：
5.6
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Wang Q;Xu J;Pu X;Lv H;Liu Y;Ma H;Wu F;Wang Q;Feng X;Liu T;Tang Q;Liu Y;Lu Y
DNA甲基化对植物的生长、发育和胁迫反应非常重要。为了了解水分胁迫（WS）下玉米根系DNA甲基化的动态变化，我们重新分析了DNA甲基化测序数据，以分析两个具有不同干旱敏感性的自交系及其衍生的两个重组自交系（RILs）的DNA甲基化和基因表达景观。结合测序基因分型技术，我们发现RILs和亲本系之间的DNA甲基化遗传模式是序列依赖的。在干旱胁迫下，玉米自交系的DNA甲基化水平明显升高，耐旱自交系的甲基化水平比干旱敏感自交系稳定得多。在不同的遗传背景中发现了不同的差异甲基化基因，这表明具有不同干旱敏感性的自交系可能以不同的方式对胁迫作出反应。基因体DNA甲基化与基因表达呈负相关，与外显子剪接事件呈正相关。此外，小干扰RNA（siRNA）与DNA甲基化之间存在不同程度的正相关关系，而这种正相关关系存在于不同的基因区域。在WS下siRNA的响应与差异DNA甲基化一致。总之，我们的数据可以是有用的，在破译DNA甲基化在植物耐旱性变化的作用，并强调其在选择性剪接的功能。
DNA methylation is important for plant growth, development, and stress response. To understand DNA methylation dynamics in maize roots under water stress (WS), we reanalyzed DNA methylation sequencing data to profile DNA methylation and the gene expression landscape of two inbred lines with different drought sensitivities, as well as two of their derived recombination inbred lines (RILs). Combined with genotyping-by-sequencing, we found that the inheritance pattern of DNA methylation between RILs and parental lines was sequence-dependent. Increased DNA methylation levels were observed under WS and the methylome of drought-tolerant inbred lines were much more stable than that of the drought-sensitive inbred lines. Distinctive differentially methylated genes were found among diverse genetic backgrounds, suggesting that inbred lines with different drought sensitivities may have responded to stress in varying ways. Gene body DNA methylation showed a negative correlation with gene expression but a positive correlation with exon splicing events. Furthermore, a positive correlation of a varying extent was observed between small interfering RNA (siRNA) and DNA methylation, which at different genic regions. The response of siRNAs under WS was consistent with the differential DNA methylation. Taken together, our data can be useful in deciphering the roles of DNA methylation in plant drought-tolerance variations and in emphasizing its function in alternative splicing.
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与干旱对比和盐度应激反应的水稻品种中与基因表达相关的不同DNA甲基化模式。
影响因子：
4.6
作者：
Garg R;Narayana Chevala V;Shankar R;Jain M
通讯作者：
Jain M
影响因子：
7.7
作者：
Dubin MJ;Zhang P;Meng D;Remigereau MS;Osborne EJ;Paolo Casale F;Drewe P;Kahles A;Jean G;Vilhjálmsson B;Jagoda J;Irez S;Voronin V;Song Q;Long Q;Rätsch G;Stegle O;Clark RM;Nordborg M
通讯作者：
Nordborg M
影响因子：
5.6
作者：
Bartels A;Han Q;Nair P;Stacey L;Gaynier H;Mosley M;Huang QQ;Pearson JK;Hsieh TF;An YC;Xiao W
通讯作者：
Xiao W
影响因子：
64.5
作者：
Kawakatsu T;Huang SC;Jupe F;Sasaki E;Schmitz RJ;Urich MA;Castanon R;Nery JR;Barragan C;He Y;Chen H;Dubin M;Lee CR;Wang C;Bemm F;Becker C;O'Neil R;O'Malley RC;Quarless DX;1001 Genomes Consortium;Schork NJ;Weigel D;Nordborg M;Ecker JR
通讯作者：
Ecker JR
DOI：
10.1073/pnas.1402275111
发表时间：
2014-06-10
影响因子：
11.1
作者：
Iwasaki, Mayumi;Paszkowski, Jerzy
通讯作者：
Paszkowski, Jerzy