Polarisation-sensitive optical coherence tomography measurement of retardance in fibrosis, a non-invasive biomarker in patients with systemic sclerosis.
Polarisation-sensitive optical coherence tomography measurement of retardance in fibrosis, a non-invasive biomarker in patients with systemic sclerosis.
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DOI：
10.1038/s41598-022-06783-7
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发表时间：
2022-02-21
影响因子：
4.6
通讯作者：
Murray AK
中科院分区：
文献类型：
--
作者：
Marjanovic EJ;Sharma V;Smith L;Pinder C;Moore TL;Manning JB;Dinsdale G;Berks M;Newton VL;Wilkinson S;Dickinson MR;Herrick AL;Watson REB;Murray AK
偏振敏感光学相干断层扫描（PS-OCT）提供了一种新的，非侵入性的方法来评估皮肤纤维化的多系统疾病系统性硬化症（SSc）通过测量胶原阻滞。本研究旨在评估作为SSc生物标志物的迟滞。31例SSc患者和27例健康对照（HC）接受了PS-OCT成像。通过临床触诊（0-3级）评估“皮肤评分”。10名患者和10名年龄/性别匹配的HC的子集进行了活检和纵向成像。组织学评估包括定量表皮厚度、胶原蛋白含量（以评估纤维化）和基质金属蛋白酶（MMP）活性（原位酶谱法）。评估PS-OCT图像的表皮厚度（结构）和纤维化（延迟）。通过组织学和结构PS-OCT测量的表皮厚度之间观察到正相关性（r = 0.79; p < 0.001）。延迟为：HC平均值0.21（SD 0.21）弧度/像素; SSc皮肤评分0，0.30（0.19）;皮肤评分1，0.11（0.16）;皮肤评分2，0.06（0.12）;皮肤评分3，0.36（0.35）。对于HC/皮肤评分0-1，各组之间的纵向延迟在1周时降低，在1个月时增加; HC活检部位延迟表明瘢痕形成类似于纤维化。通过活检和皮肤评分数据确定的延迟之间的关系表明，延迟值得进一步研究作为SSC相关纤维化的合适生物标志物。
Polarisation-sensitive optical coherence tomography (PS-OCT) offers a novel, non-invasive method of assessing skin fibrosis in the multisystem disease systemic sclerosis (SSc) by measuring collagen retardance. This study aimed to assess retardance as a biomarker in SSc. Thirty-one patients with SSc and 27 healthy controls (HC) underwent PS-OCT imaging. ‘Skin score’ was assessed by clinical palpation (0–3 scale). A subset of ten patients and ten age/sex-matched HC had a biopsy and longitudinal imaging. Histological assessment included quantification of epidermal thickness, collagen content (to assess fibrosis) and matrix metalloproteinase (MMP) activity (in situ zymography). PS-OCT images were assessed for epidermal thickness (structure) and fibrosis (retardance). Positive correlation was observed between epidermal thickness as measured by histology and structural PS-OCT (r = 0.79; p < 0.001). Retardance was: HC mean 0.21 (SD 0.21) radian/pixel; SSc skin score 0, 0.30 (0.19); skin score 1, 0.11 (0.16); skin score 2, 0.06 (0.12); skin score 3, 0.36 (0.35). Longitudinal retardance decreased at one-week across groups, increasing at one-month for HC/skin score 0–1; HC biopsy site retardance suggests scarring is akin to fibrosis. Relationships identified between retardance with both biopsy and skin score data indicate that retardance warrants further investigation as a suitable biomarker for SSc-related fibrosis.
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影响因子：
2
作者：
Khanna D;Furst DE;Clements PJ;Allanore Y;Baron M;Czirjak L;Distler O;Foeldvari I;Kuwana M;Matucci-Cerinic M;Mayes M;Medsger T Jr;Merkel PA;Pope JE;Seibold JR;Steen V;Stevens W;Denton CP
通讯作者：
Denton CP
影响因子：
5.5
作者：
Aden, N.;Shiwen, X.;Stratton, R.
通讯作者：
Stratton, R.
影响因子：
5.5
作者：
Hesselstrand, R.;Scheja, A.;Akesson, A.
通讯作者：
Akesson, A.
影响因子：
27.4
作者：
Kaloudi, Olga;Bandinelli, Francesca;Matucci-Cerinic, Marco
通讯作者：
Matucci-Cerinic, Marco
影响因子：
6.5
作者：
Lo, William C. Y.;Villiger, Martin;Bouma, Brett E.
通讯作者：
Bouma, Brett E.