Minimum information to report about a flow cytometry experiment on extracellular vesicles: Communication from the ISTH SSC subcommittee on vascular biology.
Minimum information to report about a flow cytometry experiment on extracellular vesicles: Communication from the ISTH SSC subcommittee on vascular biology.
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DOI：
10.1111/jth.15540
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Nieuwland R
中科院分区：
文献类型：
--
作者：
van der Pol E;Welsh JA;Nieuwland R
细胞外囊泡流式细胞术工作组（http：www.evflowcytometry.org）由国际细胞外囊泡学会（ISEV）、国际细胞计数促进学会（ISAC）和国际血栓和止血学会（ISTH）的成员组成。这个工作组的流式细胞术专家制定的最佳实践指南有关流式细胞术检测细胞外囊泡。为了提高严谨性和标准化，该工作组于2020年在ISEV杂志Journal of Extracellular Vesicles上发表了一个框架，概述了有关细胞外囊泡流式细胞术实验（MIFlowCyt-EV）的最小信息。与此同时，一篇解释MIFlowCyt-EV的文章发表在ISAC期刊之一的Cytometry Part A上，现在将在血栓形成和止血杂志上作为SSC通讯介绍给ISTH。该SSC通信的目标是解释为什么流式细胞术正在成为表征单个细胞外囊泡的首选工具，已经确定的障碍和（大多数）通过开发程序来克服校准流式细胞仪，以及报告最小信息的相关性，以提高流式细胞仪用于表征细胞外囊泡的实验的可靠性和再现性。
The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt‐EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt‐EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.
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影响因子：
3.7
作者：
Lee, Jamie A.;Spidlen, Josef;Boyce, Keith;Cai, Jennifer;Crosbie, Nicholas;Dalphin, Mark;Furlong, Jeff;Gasparetto, Maura.;Goldberg, Michael;Goralczyk, Elizabeth M.;Hyun, Bill;Jansen, Kirstin;Kollmann, Tobias;Kong, Megan;Leif, Robert;McWeeney, Shannon;Moloshok, Thomas D.;Moore, Wayne;Nolan, Garry;Nolan, John;Nikolich-Zugich, Janko;Parrish, David;Purcell, Barclay;Qian, Yu;Selvaraj, Biruntha;Smith, Clayton;Tchuvatkina, Olga;Wertheimer, Anne;WilkinSon, Peter;Wilson, Christopher;Wood, James;Zigon, Robert;Scheuermann, Richard H.;Brinkman, Ryan R.
通讯作者：
Brinkman, Ryan R.
跨不同流式细胞仪平台的微粒枚举的标准化：多中心协作研讨会的结果。
DOI：
10.1111/jth.13514
发表时间：
2017-01
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Cointe S;Judicone C;Robert S;Mooberry MJ;Poncelet P;Wauben M;Nieuwland R;Key NS;Dignat-George F;Lacroix R
通讯作者：
Lacroix R
影响因子：
--
作者：
de Rond, Leonie;Coumans, Frank A W;van der Pol, Edwin
通讯作者：
van der Pol, Edwin
DOI：
10.1111/aji.13350
发表时间：
2021-03
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Kuiper M;van de Nes A;Nieuwland R;Varga Z;van der Pol E
通讯作者：
van der Pol E
影响因子：
10.4
作者：
Robert, S.;Poncelet, P.;Dignat-George, F.
通讯作者：
Dignat-George, F.